急性阑尾炎患者中一氧化氮、脂质过氧化和总巯基水平的变化。
Nitric oxide, lipid peroxidation and total thiol levels in acute appendicitis.
机构信息
Department of Clinical Biochemistry, Ankara Education and Research Hospital, Ankara, Turkey.
出版信息
J Clin Lab Anal. 2010;24(2):63-6. doi: 10.1002/jcla.20301.
BACKGROUND
The mechanisms involved in the pathology of acute appendicitis (AA) and the factors affecting the progression have still been investigated. Oxidative stress is one of the factors of interest. Nitric oxide (NO) and its role in AA has not been studied previously.
METHODS
Thirty-four patients who underwent operation with a perioperative diagnosis of AA and 16 age and sex-matched controls were included in the study. Serum thiobarbituric acid reactive substances (TBARS), thiol groups (SH), NO metabolites (NO(x)), and conventional inflammation markers were determined.
RESULTS
NO(x), TBARS, C-reactive protein (CRP) levels, white blood cell (WBC) count, and erythrocyte sedimentation rate (ESR) were significantly higher, and total SH was significantly lower in AA than in control group. NO(x), TBARS, and SH levels were comparable in acute phlegmonous appendicitis and advanced appendicitis. There was a significant positive correlation between NO(x) and TBARS, CRP, ESR, WBC and a significant negative correlation between NO(x) and SH.
CONCLUSIONS
Serum NO(x) levels and oxidative stress elevate in AA independent from the extent of the lesion. Increased NO may play a role in the increased oxidative stress in AA.
背景
急性阑尾炎（AA）病理涉及的机制和影响其进展的因素仍在研究中。氧化应激是一个研究热点。一氧化氮（NO）及其在 AA 中的作用尚未被研究过。
方法
本研究纳入了 34 名接受手术且术前诊断为 AA 的患者和 16 名年龄和性别匹配的对照组。测定血清硫代巴比妥酸反应物质（TBARS）、巯基（SH）、NO 代谢产物（NO(x)）和常规炎症标志物。
结果
与对照组相比，AA 组的 NO(x)、TBARS、C 反应蛋白（CRP）水平、白细胞（WBC）计数和红细胞沉降率（ESR）显著升高，总 SH 显著降低。急性蜂窝织炎和进展性阑尾炎的 NO(x)、TBARS 和 SH 水平相当。NO(x)与 TBARS、CRP、ESR、WBC 呈显著正相关，与 SH 呈显著负相关。
结论
AA 中血清 NO(x)水平和氧化应激升高与病变程度无关。增加的 NO 可能在 AA 中增加的氧化应激中发挥作用。
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