Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA.
Cell Mol Life Sci. 2010 Jul;67(13):2163-72. doi: 10.1007/s00018-010-0322-x. Epub 2010 Mar 25.
Kinetochore function is mediated through its interaction with microtubule plus ends embedded in the kinetochore outer plate. Here, we compare and evaluate current models for kinetochore microtubule attachment, beginning with a brief review of the molecular, biochemical, cellular, and structural studies upon which these models are based. The majority of these studies strongly support a model in which the kinetochore outer plate is a network of fibers that form multiple weak attachments to each microtubule, chiefly through the Ndc80 complex. Multiple weak attachments enable kinetochores to remain attached to microtubule plus ends that are continually growing and shrinking. It is unlikely that rings or "kinetochore fibrils" have a significant role in kinetochore microtubule attachment, but such entities could have a role in stabilizing attachment, modifying microtubule dynamics, and harnessing the energy released from microtubule disassembly. It is currently unclear whether kinetochores control and coordinate the dynamics of individual kinetochore microtubules.
动粒功能是通过与嵌入动粒外板的微管正端相互作用来介导的。在这里，我们将比较和评估当前的动粒微管附着模型，首先简要回顾这些模型所基于的分子、生化、细胞和结构研究。这些研究中的大多数强烈支持这样一种模型，即动粒外板是一个纤维网络，通过 Ndc80 复合物与每个微管形成多个弱附着，主要是通过 Ndc80 复合物。多个弱附着使动粒能够保持与不断生长和收缩的微管正端相连。环或“动粒原纤维”不太可能在动粒微管附着中起重要作用，但这些实体可能在稳定附着、修饰微管动力学以及利用微管解聚释放的能量方面发挥作用。目前尚不清楚动粒是否控制和协调单个动粒微管的动力学。
