Liu Hui, Zhanl Dongsheng, Du Yiqun
Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Southeast University, Nanjing, 210009, China.
J Nanosci Nanotechnol. 2010 Jan;10(1):514-9. doi: 10.1166/jnn.2010.1591.
The aim of this paper is to prepare Fe3O4 nanoparticles and study its immunotherapeutic effect as adjuvants on mice H22 live cancer. The Fe3O4 nanoparticles were prepared by chemical coprecipitation route. Transmission electron microscopy (TEM), X-ray diffraction (XRD), Energy Dispersive Analysis (EDS) were used to characterize Fe3O4 nanoparticles. The Fe3O4 nanoparticles were compared with the common alum adjuvants for its ability to induce immunity to inhibit tumor growth rate by prophylactic and therapeutic studies. Results indicated that Fe3O4 nanopaticles adsorbed autovaccine took great advantages over the common alum adjuvants after subcutaneous injection, raised the mass inhibitory rate of tumor, boosted the activity of cytotoxicity and enhanced the level of IFN-gamma cytokine. Thus, we concluded that Fe3O4 nanoparticles as adjuvants had great potential for enhancing anti-tumor immune response.
本文旨在制备Fe3O4纳米颗粒，并研究其作为佐剂对小鼠H22活癌的免疫治疗效果。通过化学共沉淀法制备Fe3O4纳米颗粒。采用透射电子显微镜（TEM）、X射线衍射（XRD）、能量色散分析（EDS）对Fe3O4纳米颗粒进行表征。通过预防性和治疗性研究，将Fe3O4纳米颗粒与普通明矾佐剂在诱导免疫抑制肿瘤生长速率的能力方面进行比较。结果表明，皮下注射后，吸附自身疫苗的Fe3O4纳米颗粒比普通明矾佐剂具有很大优势，提高了肿瘤的质量抑制率，增强了细胞毒性活性，提高了IFN-γ细胞因子水平。因此，我们得出结论，Fe3O4纳米颗粒作为佐剂在增强抗肿瘤免疫反应方面具有巨大潜力。
