Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.
PLoS One. 2010 Mar 25;5(3):e9833. doi: 10.1371/journal.pone.0009833.
Surface plasmon resonance imaging and surface plasmon induced fluorescent are sensitive tools for surface analysis. However, existing instruments in this area have provided limited capability for concurrent detection, and may be large and expensive. We demonstrate a highly cost-effective system capable of concurrent surface plasmon resonance microscopy (SPRM) and surface plasmon resonance-enhanced fluorescence (SPRF) imaging, allowing for simultaneous monitoring of reflectivity and fluorescence from discrete spatial regions. The instrument allows for high performance imaging and quantitative measurements with surface plasmon resonance, and surface plasmon induced fluorescence, with inexpensive off-the-shelf components.
表面等离子体共振成像和表面等离子体诱导荧光是用于表面分析的敏感工具。然而，该领域现有的仪器在同时检测方面提供的功能有限，而且可能体积大且昂贵。我们展示了一种具有成本效益的系统，能够同时进行表面等离子体共振显微镜 (SPRM) 和表面等离子体共振增强荧光 (SPRF) 成像，允许从离散的空间区域同时监测反射率和荧光。该仪器使用廉价的现成组件，实现了具有表面等离子体共振和表面等离子体诱导荧光的高性能成像和定量测量。
