Department of Food and Nutrition and Korean Institute of Nutrition, Hallym University, Chuncheon, Korea.
Nutr Res Pract. 2007 Winter;1(4):279-84. doi: 10.4162/nrp.2007.1.4.279. Epub 2007 Dec 31.
Solar ultraviolet (UV) irradiation leads to distinct changes in the skin connective tissues by degradation of collagen, which is a major structural component in the extracellular matrix. UV irradiation induces the production of matrix metalloproteinases (MMP) capable of attacking native fibrillar collagen and responsible for inhibiting the construction of collagenous extracellular matrix. In this study, we attempted to investigate the protective actions of Rubus coreanus ethanol extract (RCE) on the MMP production and the consequent procollagen/collagen degradation in UV-B-irradiated human dermal fibroblasts. The analytical data showed that Rubus coreanus ethanol extract was mostly comprised of cyanidin 3-rutinoside. Pre-treatment of fibroblasts with this extract inhibited UV-B-induced production of MMP-1, MMP-8 and MMP-13 in dose-dependent manners. In addition, Western blot analysis and immunocytochemical staining assay revealed that RCE markedly augmented the cellular levels of procollagen/collagen declined in UV-B-exposed dermal fibroblasts. These results demonstrate that RCE blocks UV-B-induced increase of the collagen degradation by inhibiting MMP production. Thus, RCE may act as an agent inhibiting excessive dermal collagen degradation leading to the skin photoaging.
阳光中的紫外线（UV）照射会导致皮肤结缔组织发生明显变化，使胶原蛋白降解，而胶原蛋白是细胞外基质的主要结构成分。UV 照射会诱导基质金属蛋白酶（MMP）的产生，这些酶能够攻击天然的纤维状胶原蛋白，从而抑制胶原蛋白细胞外基质的构建。在这项研究中，我们试图研究覆盆子乙醇提取物（RCE）对 MMP 产生的保护作用，以及随之而来的 UV-B 照射下人真皮成纤维细胞中原发性胶原/胶原蛋白的降解。分析数据表明，覆盆子乙醇提取物主要由矢车菊素 3-芸香糖苷组成。该提取物预处理真皮成纤维细胞，可呈剂量依赖性抑制 MMP-1、MMP-8 和 MMP-13 的产生。此外，Western blot 分析和免疫细胞化学染色试验表明，RCE 显著增加了 UV-B 暴露的真皮成纤维细胞中下降的原胶原蛋白/胶原蛋白的细胞水平。这些结果表明，RCE 通过抑制 MMP 的产生来阻止 UV-B 诱导的胶原蛋白降解增加。因此，RCE 可能作为一种抑制皮肤光老化导致的过度真皮胶原降解的药物。
