Department of Preventive Medicine, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Evid Based Complement Alternat Med. 2012;2012:545497. doi: 10.1155/2012/545497. Epub 2012 Dec 5.
KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.
KM110329 是四种具有抗炎特性的传统草药混合物。特应性皮炎（AD）是一种炎症性皮肤病，与过敏原引起的辅助性 T 细胞 2（Th2）淋巴细胞反应增强有关，导致血清嗜酸性粒细胞和免疫球蛋白 E（IgE）水平升高，以及特应性皮肤部位的白细胞浸润。在这项研究中，我们研究了 KM110329 乙醇提取物对卵清蛋白（OVA）或 2,4-二硝基氯苯-（DNCB-）诱导的 AD 小鼠模型的影响。为此，我们观察了 KM110329 对 OVA 过敏原或 DNCB 处理的 BALB/c 小鼠的血液嗜酸性粒细胞、皮肤肥大细胞、血清 IgE 产生和特应性皮炎皮肤病变中细胞因子 mRNA 表达的影响。KM110329 显著减少了 OVA 或 DNCB 处理的 BALB/c 小鼠的血液嗜酸性粒细胞数量。组织学分析表明，肥大细胞计数以及炎症细胞的真皮浸润减少。在皮肤病变中，KM110329 抑制了白细胞介素（IL）-4、IL-13 和 IL-17 的 mRNA 表达。KM110329 还抑制了 OVA 和 DNCB 诱导的特应性皮炎模型中血清 IgE 水平的产生。总之，我们的研究结果表明，KM110329 提取物的局部应用对 AD 症状有有益的影响，表明 KM110329 可能是治疗 AD 的有用候选药物。
