Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal;
Persoonia. 2008 Dec;21:29-55. doi: 10.3767/003158508X340742. Epub 2008 Jul 16.
Species in the Botryosphaeriaceae are common plant pathogens and saprobes found on a variety of mainly woody hosts. Teleomorphs typically have hyaline, aseptate ascospores. However, some have been reported with brown ascospores and their taxonomic status is uncertain. A multi-gene approach (SSU, ITS, LSU, EF1-alpha and beta-tubulin) was used to resolve the correct phylogenetic position of the dark-spored 'Botryosphaeria' teleomorphs and related asexual species. Neodeightonia and Phaeobotryon are reinstated for species with brown ascospores that are either 1-septate (Neodeightonia) or 2-septate (Phaeobotryon). Phaeobotryosphaeria is reinstated for species with brown, aseptate ascospores that bear an apiculus at either end. The status of Sphaeropsis is clarified and shown to be the anamorph of Phaeobotryosphaeria. Two new genera, namely Barriopsis for species having brown, aseptate ascospores without apiculi and Spencermartinsia for species having brown, 1-septate ascospores with an apiculus at either end are introduced. Species of Dothiorella have brown, 1-septate ascospores and differ from Spencermartinsia in the absence of apiculi. These six genera can also be distinguished from one another based on morphological characters of their anamorphs. Although previously placed in the Botryosphaeriaceae, Dothidotthia, was shown to belong in the Pleosporales, and the new family Dothidotthiaceae is introduced to accommodate it.
子囊菌门 Botryosphaeriaceae 中的物种是常见的植物病原体和腐生物，存在于多种主要的木本宿主上。其有性型通常具有透明、无隔的子囊孢子。然而，有些种已被报道具有棕色子囊孢子，其分类地位尚不确定。本研究采用多基因方法（SSU、ITS、LSU、EF1-α和β-微管蛋白）来解决深色孢子“Botryosphaeria”有性型及其相关无性型的正确系统发育位置。Neodeightonia 和 Phaeobotryon 被重新确立，用于具有棕色子囊孢子的种，这些孢子要么是 1 隔（Neodeightonia），要么是 2 隔（Phaeobotryon）。Phaeobotryosphaeria 被重新确立，用于具有棕色、无隔子囊孢子且两端具顶生附属丝的种。Sphaeropsis 的地位得到澄清，并被证明是 Phaeobotryosphaeria 的无性型。引入了两个新属，即 Barriopsis，用于具有棕色、无隔子囊孢子且无附属丝的种，以及 Spencermartinsia，用于具有棕色、1 隔子囊孢子且两端具顶生附属丝的种。Dothiorella 属的种具有棕色、1 隔子囊孢子，与 Spencermartinsia 的区别在于没有附属丝。这些六个属还可以根据它们无性型的形态特征彼此区分。虽然以前被置于 Botryosphaeriaceae 中，但 Dothidotthia 被证明属于 Pleosporales，引入了新的科 Dothidotthiaceae 来容纳它。
