Department of Biological Sciences, University of Warwick, Coventry, UK.
Influenza Other Respir Viruses. 2010 May 1;4(3):147-53. doi: 10.1111/j.1750-2659.2010.00130.x.
The cell surface receptor used by an influenza virus to infect that cell is an N-acetyl neuraminic acid (NANA) residue terminally linked by an alpha2,3 or alpha2,6 bond to a carbohydrate moiety of a glycoprotein or glycolipid. Our aim was to determine a quick and technically simple method to determine cell receptor usage by whole influenza A virus particles.
We employed surface plasmon resonance to detect the binding of viruses to fetuin, a naturally occurring glycoprotein that has both alpha2,3- and alpha2,6-linked NANA, and free 3'-sialyllactose or 6'-sialyllactose to compete virus binding. All virus stocks were produced in embryonated chicken's eggs.
The influenza viruses tested bound preferentially to NANAalpha2,3Gal or to NANAalpha2,6Gal, or showed no preference. Two PR8 viruses had different binding preferences. Binding preferences of viruses correlated well with their known biological properties.
Our data suggest that it is not easy to predict receptor usage by influenza viruses. However, direct experimental determination as described here can inform experiments concerned with viral pathogenesis, biology and structure. In principle, the methodology can be used for any virus that binds to a terminal NANA residue.
流感病毒感染细胞所使用的细胞表面受体是末端通过α2,3 或 α2,6 键与糖蛋白或糖脂的碳水化合物部分连接的 N-乙酰神经氨酸（NANA）残基。我们的目的是确定一种快速且技术简单的方法来确定全流感 A 病毒颗粒的细胞受体用途。
我们采用表面等离子体共振来检测病毒与胎球蛋白的结合，胎球蛋白是一种天然存在的糖蛋白，具有 α2,3-和 α2,6-连接的 NANA 以及游离的 3'-唾液酸乳糖或 6'-唾液酸乳糖，以竞争病毒结合。所有病毒株均在鸡胚中产生。
测试的流感病毒优先结合 NANAα2,3Gal 或 NANAα2,6Gal，或者没有偏好。两种 PR8 病毒具有不同的结合偏好。病毒的结合偏好与其已知的生物学特性密切相关。
我们的数据表明，预测流感病毒的受体用途并不容易。然而，如这里所述的直接实验测定可以为与病毒发病机制、生物学和结构相关的实验提供信息。原则上，该方法可用于任何与末端 NANA 残基结合的病毒。
