Arvieux J, Roussel B, Ponard D, Colomb M G
Laboratories d'Immunologie, Centre de Transfusion Sanguine, France.
Clin Exp Immunol. 1991 Jun;84(3):466-71.
We studied 51 sera from patients with systemic lupus erythematosus to determine the relationship of their anti-phospholipid activity to their anti-erythrocyte and complement activation properties. Forty-nine per cent of the sera had anti-phospholipid activity as demonstrated by ELISA using a panel of anionic phospholipids, and most of these also bound to neutral phospholipids, albeit to a lesser extent. A cellular radioimmunoassay was used for the detection of immunoglobulin and C3 binding to normal erythrocytes (intact, enzyme-treated or glutaraldehyde-fixed) following incubation with patient sera. The levels of IgG anti-phospholipid correlated with hypocomplementaemia and with immunoglobulin binding (paralleled by a deposition of C3 fragments) to the three types of erythrocytes, although most strongly to fixed cells. Immunoglobulin binding to intact erythrocytes correlated primarily with reactivity against neutral phospholipids. The specificity for phospholipid epitopes of immunoglobulin adsorbed onto erythrocytes was confirmed by acid elution followed by testing in ELISA. These data suggest that some anti-phospholipid antibody subsets may bind to erythrocytes in vivo, thus accounting for the observed association of these antibodies with positive direct antiglobulin tests.
我们研究了51份系统性红斑狼疮患者的血清,以确定其抗磷脂活性与其抗红细胞及补体激活特性之间的关系。使用一组阴离子磷脂通过酶联免疫吸附测定(ELISA)证明,49%的血清具有抗磷脂活性,并且其中大多数血清也能与中性磷脂结合,尽管结合程度较低。采用细胞放射免疫测定法检测患者血清与正常红细胞(完整、酶处理或戊二醛固定)孵育后免疫球蛋白和C3的结合情况。IgG抗磷脂水平与补体减少以及免疫球蛋白与三种类型红细胞的结合(伴有C3片段沉积)相关,尽管与固定细胞的相关性最强。免疫球蛋白与完整红细胞的结合主要与对中性磷脂的反应性相关。通过酸洗脱后在ELISA中进行检测,证实了吸附在红细胞上的免疫球蛋白对磷脂表位的特异性。这些数据表明,某些抗磷脂抗体亚群可能在体内与红细胞结合,从而解释了这些抗体与直接抗球蛋白试验阳性之间的关联。