Scheel O, Anestad G, Mundal R, Berdal B P
Department of Clinical Microbiology, Central Hospital of Esbjerg, Denmark.
Eur J Clin Microbiol Infect Dis. 1993 Oct;12(10):746-9. doi: 10.1007/BF02098461.
First-void urine samples from 392 Norwegian military conscripts were investigated for the presence of Chlamydia trachomatis by enzyme immunoassay (EIA) on day 1 and day 5 after collection. Positive samples were subsequently investigated by direct immunofluorescence (IF) microscopy for the presence of chlamydial elementary bodies (EBs) in the urine pellet, and urethral swab material taken from the EIA-positive individuals was cultured. 4.8% (19/392) of the urine samples were EIA-positive on day 1, and 5.4% (21/392) were positive on day 5, with a combined total of 6.6% (26/392). Twenty-four of the 26 urine samples were confirmed as positive on IF microscopy. Urethral swabs were taken from 21 EIA-positive individuals. Six of the swabs were positive on cell culture, whereas nine were positive on IF microscopy of swab material, suggesting that these techniques perform better in symptomatic cases than in male Chlamydia trachomatis carriers. In the urine samples a notable discrepancy in EIA results was seen when the same refrigerated samples were retested on day 5 compared to day 1. This discrepancy was probably due to storage-related factors.
对392名挪威应征入伍者采集的首次晨尿样本在采集后第1天和第5天通过酶免疫测定法（EIA）检测沙眼衣原体的存在情况。随后对阳性样本进行直接免疫荧光（IF）显微镜检查，以检测尿沉渣中衣原体原体（EBs）的存在，并对EIA阳性个体采集的尿道拭子样本进行培养。第1天，4.8%（19/392）的尿样EIA呈阳性，第5天为5.4%（21/392），两者合计为6.6%（26/392）。26份尿样中有24份经IF显微镜检查确认为阳性。从21名EIA阳性个体采集了尿道拭子。其中6份拭子在细胞培养中呈阳性，而9份在拭子样本的IF显微镜检查中呈阳性，这表明这些技术在有症状的病例中比在男性沙眼衣原体携带者中表现更好。在尿样中，当在第5天对相同的冷藏样本重新检测时，与第1天相比，EIA结果存在显著差异。这种差异可能是由于与储存相关的因素。
