Department of Dermatology and Allergology, Friedrich Schiller University, Jena, Germany.
Skin Res Technol. 2010 Aug;16(3):259-64. doi: 10.1111/j.1600-0846.2010.00437.x.
The in vivo measurement of epidermal thickness is still challenging. While ultrasound, optical coherence tomography and confocal laser microscopy are used with moderate success, this issue has not been addressed by multiphoton laser tomography.
In the present study, an in vivo measurement of four different morphometric epidermal parameters is performed.
Thirty healthy volunteers aged 21-82 years were included in the study after informed consent and approval of the local ethics committee. At the dorsal forearm and the dorsum of the hand, the thicknesses of the total epidermis, viable epidermis and stratum corneum and the depth of the papillary dermis were calculated from depth-resolved intensity curves after correlation with multiphoton images.
We have shown consistently that in all age groups, the four morphometric parameters are significantly higher at the hand compared with the forearm, while there were no differences between age groups. This is consistent with most previous findings.
The method presented here provides a novel in vivo investigation tool for the measurement of epidermal morphometric parameters that may be useful for the observation of epidermal changes over time in skin disorders, therapy side effects or in cosmetic science.
目前，活体测量表皮厚度仍然具有挑战性。尽管超声、光学相干断层扫描和共聚焦激光显微镜已经取得了一定的成功，但多光子激光断层扫描并未解决这一问题。
本研究旨在对四种不同的表皮形态参数进行活体测量。
在获得知情同意并获得当地伦理委员会批准后，本研究纳入了 30 名年龄在 21-82 岁之间的健康志愿者。在手背和手掌背部，通过与多光子图像相关联，从深度分辨强度曲线中计算出总表皮、有活力的表皮和角质层的厚度以及乳头真皮的深度。
我们一致表明，在所有年龄组中，这四个形态参数在手背部均显著高于前臂，而不同年龄组之间无差异。这与大多数先前的发现一致。
本文提出的方法为测量表皮形态参数提供了一种新的活体研究工具，对于观察皮肤疾病、治疗副作用或美容科学中表皮随时间的变化可能具有重要意义。
