IDH1 和 p53 在骨肉瘤中的表达及意义。

The expression and significance of IDH1 and p53 in osteosarcoma.

机构信息

Department of Orthopedics, Zhongnan Hospital of Wuhan University, No 169 Donghu Road, Wuchang District, 430071, Wuhan, China.

出版信息

J Exp Clin Cancer Res. 2010 May 7;29(1):43. doi: 10.1186/1756-9966-29-43.

BACKGROUND

To detect the expression of isocitrate dehydrogenase 1 (IDH1) and transformation-related protein 53 (p53) in osteosarcoma and analyze the correlation between them and the clinico-pathological features.

METHODS

The expressions of IDH1 and p53 were detected in human osteosarcoma cell lines (MG-63 and U2OS) by immunocytochemistry, Real-time PCR and Western Blotting. The expressions of IDH1 and p53 in formalin-fixed paraffin-embedded tissue sections from 44 osteosarcoma patients were determined by immunohistochemistry, and the correlation between them and clinicopagthological features were analyzed. None of these patients received chemotherapy prior to surgery.

RESULTS

IDH1 is detected in osteosarcoma cell lines and biopsies. IDH1 expresses higher in U2OS cells with wild type p53 than in MG-63 cells with mutation p53. IDH1 correlates with histological Rosen grade and metastasis negatively. P53 correlates with histological Rosen grade, metastasis and overall survival in clinical osteosarcoma biopsies. Osteosarcoma patients with High IDH1 expression have a very high p53 expression.

CONCLUSION

IDH1 may correlate with p53 and be a candidate biomarker for osteosarcoma correlate with histological Rosen grade and metastasis.

背景

检测异柠檬酸脱氢酶 1（IDH1）和转化相关蛋白 53（p53）在骨肉瘤中的表达，并分析它们与临床病理特征的相关性。

方法

采用免疫细胞化学、实时 PCR 和 Western blot 检测人骨肉瘤细胞系（MG-63 和 U2OS）中 IDH1 和 p53 的表达。采用免疫组织化学检测 44 例骨肉瘤患者福尔马林固定石蜡包埋组织切片中 IDH1 和 p53 的表达，并分析它们与临床病理特征的相关性。这些患者在手术前均未接受化疗。

结果

IDH1 在骨肉瘤细胞系和活检中均有检测到。在野生型 p53 的 U2OS 细胞中，IDH1 的表达高于突变型 p53 的 MG-63 细胞。IDH1 与组织学 Rosen 分级和转移呈负相关。p53 与临床骨肉瘤活检中的组织学 Rosen 分级、转移和总生存率相关。IDH1 高表达的骨肉瘤患者 p53 表达也非常高。