Department of Chemical Engineering, University of Washington, Box 355014, Seattle, WA 98195, USA.
Biotechnol Lett. 2010 Oct;32(10):1351-9. doi: 10.1007/s10529-010-0306-9. Epub 2010 May 21.
A brief historical perspective on metagenomics is given followed by a discussion of the rapid progress in this field largely defined by transition to the next generation sequencing technologies. Problems and challenges connected to this transition are also addressed. The review focuses on recent literature describing metagenomic approaches connecting sequence information to functionality that are especially relevant to biotechnological applications, including metagenomics of specialized or enriched microbial communities, metagenomics combined with specific labeling techniques, metatranscriptomics and metaproteomics.
本文简要回顾了宏基因组学的历史发展，主要讨论了该领域在向新一代测序技术转变过程中取得的快速进展。文中还讨论了这一转变所带来的问题和挑战。本文重点关注了最近的文献，这些文献描述了将序列信息与功能联系起来的宏基因组学方法，这些方法尤其与生物技术应用相关，包括特殊或富集微生物群落的宏基因组学、与特定标记技术相结合的宏基因组学、宏转录组学和宏蛋白质组学。
