活体超分辨成像观察斜光刺激诱导的瞬时视网膜光向性
In vivo super-resolution imaging of transient retinal phototropism evoked by oblique light stimulation.
机构信息
University of Illinois at Chicago, Department of Bioengineering, Chicago, Illinois, United States.
University of Illinois at Chicago, Department of Ophthalmology and Visual Sciences, Chicago, Illinoi, United States.
出版信息
J Biomed Opt. 2018 May;23(5):1-4. doi: 10.1117/1.JBO.23.5.050502.
Abstract
Rod-dominated transient retinal phototropism (TRP) has been observed in freshly isolated retinas, promising a noninvasive biomarker for objective assessment of retinal physiology. However, in vivo mapping of TRP is challenging due to its subcellular signal magnitude and fast time course. We report here a virtually structured detection-based super-resolution ophthalmoscope to achieve subcellular spatial resolution and millisecond temporal resolution for in vivo imaging of TRP. Spatiotemporal properties of in vivo TRP were characterized corresponding to variable light intensity stimuli, confirming that TRP is tightly correlated with early stages of phototransduction.
摘要
杆状细胞主导的瞬时视网膜光偏向性（TRP）已在新鲜离体视网膜中观察到，这为客观评估视网膜生理提供了一种非侵入性的生物标志物。然而，由于其亚细胞信号幅度和快速时间过程，体内 TRP 的映射具有挑战性。我们在这里报告了一种基于虚拟结构检测的超分辨率眼底镜，以实现亚细胞空间分辨率和毫秒时间分辨率，用于体内 TRP 的成像。与可变光强刺激相对应的体内 TRP 的时空特性进行了特征描述，证实 TRP 与光转导的早期阶段密切相关。
相似文献
1
In vivo super-resolution imaging of transient retinal phototropism evoked by oblique light stimulation.
J Biomed Opt. 2018 May;23(5):1-4. doi: 10.1117/1.JBO.23.5.050502.
2
observation of transient photoreceptor movement correlated with oblique light stimulation.
Proc SPIE Int Soc Opt Eng. 2018 Jan-Feb;10497. doi: 10.1117/12.2287262. Epub 2018 Feb 20.
3
Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging.
Exp Biol Med (Maywood). 2021 Feb;246(3):249-259. doi: 10.1177/1535370220970533. Epub 2020 Nov 27.
4
Dynamic near-infrared imaging reveals transient phototropic change in retinal rod photoreceptors.
J Biomed Opt. 2013 Oct;18(10):106013. doi: 10.1117/1.JBO.18.10.106013.
5
In vivo confocal imaging of fast intrinsic optical signals correlated with frog retinal activation.
Opt Lett. 2011 Dec 1;36(23):4692-4. doi: 10.1364/OL.36.004692.
6
Biophysical mechanism of transient retinal phototropism in rod photoreceptors.
Proc SPIE Int Soc Opt Eng. 2016 Feb 13;9706. doi: 10.1117/12.2209144. Epub 2016 Mar 7.
7
In vivo confocal intrinsic optical signal identification of localized retinal dysfunction.
Invest Ophthalmol Vis Sci. 2012 Dec 13;53(13):8139-45. doi: 10.1167/iovs.12-10732.
8
Stimulus-evoked outer segment changes in rod photoreceptors.
J Biomed Opt. 2016 Jun 1;21(6):65006. doi: 10.1117/1.JBO.21.6.065006.
9
In vivo super-resolution retinal imaging through virtually structured detection.
J Biomed Opt. 2016 Dec 1;21(12):120502. doi: 10.1117/1.JBO.21.12.120502.
10
Functional imaging of retinal photoreceptors and inner neurons using stimulus-evoked intrinsic optical signals.
Methods Mol Biol. 2012;884:277-85. doi: 10.1007/978-1-61779-848-1_20.
引用本文的文献
1
Functional Optical Coherence Tomography for Intrinsic Signal Optoretinography: Recent Developments and Deployment Challenges.
Front Med (Lausanne). 2022 Apr 4;9:864824. doi: 10.3389/fmed.2022.864824. eCollection 2022.
2
Virtually structured detection enables super-resolution ophthalmoscopy of rod and cone photoreceptors in human retina.
Quant Imaging Med Surg. 2021 Mar;11(3):1060-1069. doi: 10.21037/qims-20-542.
3
Super-resolution ophthalmoscopy: Virtually structured detection for resolution improvement in retinal imaging.
Exp Biol Med (Maywood). 2021 Feb;246(3):249-259. doi: 10.1177/1535370220970533. Epub 2020 Nov 27.
4
Functional optical coherence tomography enables optoretinography of photoreceptor dysfunction due to retinal degeneration.
Biomed Opt Express. 2020 Aug 27;11(9):5306-5320. doi: 10.1364/BOE.399334. eCollection 2020 Sep 1.
5
Fast intrinsic optical signal correlates with activation phase of phototransduction in retinal photoreceptors.
Exp Biol Med (Maywood). 2020 Jul;245(13):1087-1095. doi: 10.1177/1535370220935406. Epub 2020 Jun 19.
6
Light-Induced Length Shrinkage of Rod Photoreceptor Outer Segments.
Transl Vis Sci Technol. 2018 Dec 21;7(6):29. doi: 10.1167/tvst.7.6.29. eCollection 2018 Nov.
7
Functional optical coherence tomography of retinal photoreceptors.
Exp Biol Med (Maywood). 2018 Dec;243(17-18):1256-1264. doi: 10.1177/1535370218816517. Epub 2018 Nov 27.
本文引用的文献
1
observation of transient photoreceptor movement correlated with oblique light stimulation.
Proc SPIE Int Soc Opt Eng. 2018 Jan-Feb;10497. doi: 10.1117/12.2287262. Epub 2018 Feb 20.
2
Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors.
Biomed Opt Express. 2016 Dec 2;8(1):38-47. doi: 10.1364/BOE.8.000038. eCollection 2017 Jan 1.
3
In vivo super-resolution retinal imaging through virtually structured detection.
J Biomed Opt. 2016 Dec 1;21(12):120502. doi: 10.1117/1.JBO.21.12.120502.
4
In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas.
J Biomed Opt. 2016 Sep 1;21(9):96010. doi: 10.1117/1.JBO.21.9.096010.
5
Stimulus-evoked outer segment changes in rod photoreceptors.
J Biomed Opt. 2016 Jun 1;21(6):65006. doi: 10.1117/1.JBO.21.6.065006.
6
Intrinsic optical signal imaging of retinal physiology: a review.
J Biomed Opt. 2015 Sep;20(9):090901. doi: 10.1117/1.JBO.20.9.090901.
7
Functional optical coherence tomography enables in vivo physiological assessment of retinal rod and cone photoreceptors.
Sci Rep. 2015 Apr 22;5:9595. doi: 10.1038/srep09595.
8
Comparison of Visual Function in Older Eyes in the Earliest Stages of Age-related Macular Degeneration to Those in Normal Macular Health.
Curr Eye Res. 2016;41(2):266-72. doi: 10.3109/02713683.2015.1011282. Epub 2015 Mar 24.
9
Line-field parallel swept source MHz OCT for structural and functional retinal imaging.
Biomed Opt Express. 2015 Feb 4;6(3):716-35. doi: 10.1364/BOE.6.000716. eCollection 2015 Mar 1.
10
Functional optical coherence tomography reveals transient phototropic change of photoreceptor outer segments.
Opt Lett. 2014 Dec 15;39(24):6923-6. doi: 10.1364/OL.39.006923.
Zotero 插件