Department of Human Biology, Maastricht University Medical Centre, Maastricht, The Netherlands. M.Geraedts @ HB.unimaas.nl
Ann Nutr Metab. 2010;56(4):308-13. doi: 10.1159/000312664. Epub 2010 Jun 8.
BACKGROUND/AIM: High protein diets are the most effective to stimulate cholecystokinin (CCK) and glucagon-like peptide 1 (GLP-1) release; however, which proteins are the most potent is not known. Here, the effects of specific dietary proteins on intestinal CCK and GLP-1 release were examined.
Duodenal biopsies of 10 healthy male subjects and 10 male rats were taken and placed in an Ussing chamber system. The biopsies were exposed on the luminal side to buffer, egg protein, codfish protein, ovomucoid, pea protein, and wheat protein. After an exposure time of 2 h, samples were taken from the serosal side.
Pea protein and wheat protein increased CCK and GLP-1 release in human duodenal tissue, while codfish protein only increased CCK release. No elevated levels of CCK and GLP-1 were found after exposure of rat tissue to different proteins.
Pea and wheat protein are the most potent stimulators of CCK and GLP-1 release in human duodenal tissue, and may therefore be good dietary additives in weight management.
背景/目的：高蛋白饮食最能有效地刺激胆囊收缩素（CCK）和胰高血糖素样肽 1（GLP-1）的释放；然而，哪种蛋白质的效果最强尚不清楚。在这里，研究了特定膳食蛋白质对肠道 CCK 和 GLP-1 释放的影响。
从 10 名健康男性受试者和 10 只雄性大鼠中取出十二指肠活检样本，并将其放置在 Ussing 室系统中。将活检样本暴露于腔内缓冲液、卵清蛋白、鳕鱼蛋白、卵转铁蛋白、豌豆蛋白和小麦蛋白上。暴露 2 小时后，从浆膜侧取样。
豌豆蛋白和小麦蛋白增加了人十二指肠组织中的 CCK 和 GLP-1 释放，而鳕鱼蛋白仅增加了 CCK 释放。暴露于不同蛋白质后，大鼠组织中未发现 CCK 和 GLP-1 水平升高。
豌豆蛋白和小麦蛋白是刺激人十二指肠组织中 CCK 和 GLP-1 释放的最有效物质，因此可能是体重管理的良好膳食添加剂。
