EMBL-CRG Systems Biology Program; Centre for Genomic Regulation; UPF; Barcelona, Spain; Istituciô Catalana de Recerca i Estudis Avançats; Barcelona, Spain.
Organogenesis. 2009 Oct;5(4):211-6. doi: 10.4161/org.5.4.10426.
Optical projection tomography (OPT) is a technology ideally suited for imaging embryonic organs. We emphasize here recent successes in translating this potential into the field of live imaging. Live OPT (also known as 4D OPT, or time-lapse OPT) is already in position to accumulate good quantitative data on the developmental dynamics of organogenesis, a prerequisite for building realistic computer models and tackling new biological problems. Yet, live OPT is being further developed by merging state-of-the-art mouse embryo culture with the OPT system. We discuss the technological challenges that this entails and the prospects for expansion of this molecular imaging technique into a wider range of applications.
光学投影断层成像术（OPT）是一种非常适合于对胚胎器官成像的技术。我们在此强调最近在将这种潜力转化为活体成像领域的成功。活体 OPT（也称为 4D OPT 或延时 OPT）已经能够积累有关器官发生发育动力学的良好定量数据，这是建立现实计算机模型和解决新生物学问题的前提。然而，活体 OPT 通过将最先进的小鼠胚胎培养与 OPT 系统相结合而得到进一步发展。我们讨论了这所需要的技术挑战以及将这种分子成像技术扩展到更广泛应用的前景。
