Charité - Universitätsmedizin Berlin, Center of Experimental and Cutaneous Physiology, Berlin, Germany.
Eur J Pharm Biopharm. 2010 Oct;76(2):269-74. doi: 10.1016/j.ejpb.2010.06.004. Epub 2010 Jun 15.
Skin functions and structure are significantly influenced by nutrients. Antioxidants protect the supportive layer of the skin against any damaging irradiation effects and the action of free radicals. A lack of suitable methods means that the pharmacokinetic properties of systemically applied carotenoids transferred into the skin remain poorly understood. In this study, a natural kale extract or placebo oil were given orally to 22 healthy volunteers for 4 weeks. Carotenoid bioaccessibility was evaluated using non-invasive resonance Raman spectroscopy on the palm and forehead skin. For the analysis of the blood serum, the standard HPLC method was used. The blood and skin levels of the carotenoids increased significantly during the study but compared to the blood serum values, increases in skin were delayed and depended on the dermal area as well as on the carotenoid. Lycopene, measured as being low in the extract, increases more in the skin compared to the blood indicating that the natural mixture of the extract stabilizes the antioxidative network in the skin. After supplementation had ended, the carotenoids decreased much faster in the blood than in the skin. The delayed decrease in the skin may indicate a peripheral buffer function of the skin for carotenoids.
皮肤的功能和结构受到营养物质的显著影响。抗氧化剂可保护皮肤的支撑层免受任何有害辐射影响和自由基的作用。由于缺乏合适的方法，全身应用的类胡萝卜素转移到皮肤中的药代动力学特性仍知之甚少。在这项研究中，22 名健康志愿者口服天然羽衣甘蓝提取物或安慰剂油 4 周。使用非侵入式共振拉曼光谱法在手掌和额头上的皮肤评估类胡萝卜素的生物利用度。为了分析血清，使用了标准的 HPLC 方法。在研究过程中，血液和皮肤中的类胡萝卜素水平显著增加，但与血清值相比，皮肤中的增加延迟，并且取决于真皮面积和类胡萝卜素。番茄红素在提取物中含量较低，在皮肤中的增加量高于血液，表明提取物中的天然混合物稳定了皮肤中的抗氧化网络。补充结束后，类胡萝卜素在血液中的下降速度比在皮肤中快得多。皮肤中类胡萝卜素的下降延迟可能表明皮肤具有类胡萝卜素的外周缓冲功能。
