免疫荧光法与酶免疫法检测麻疹特异性免疫球蛋白M抗体的比较
Comparison of immunofluorescence and enzyme immunoassay for detection of measles-specific immunoglobulin M antibody.
作者信息
Rossier E, Miller H, McCulloch B, Sullivan L, Ward K
机构信息
Department of Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
出版信息
J Clin Microbiol. 1991 May;29(5):1069-71. doi: 10.1128/jcm.29.5.1069-1071.1991.
Abstract
During a measles outbreak, 283 serum specimens from 221 suspected cases of measles were tested by immunofluorescence and enzyme immunoassay for the presence of measles-specific immunoglobulin M (IgM) antibodies by using commercially available reagents. There was 97% agreement between the two assays; thus, the choice of the method for diagnostic testing is a matter of convenience and experience. In all 62 cases of measles from which a single blood sample was available, measles IgM-specific antibodies were detectable by both methods. Fifty percent of the 62 cases were positive within 3 days after onset of the rash. This increased to 91% 10 days after onset of the rash.
摘要
在一次麻疹疫情期间，使用市售试剂通过免疫荧光法和酶免疫法对来自221例疑似麻疹病例的283份血清标本进行检测，以确定是否存在麻疹特异性免疫球蛋白M（IgM）抗体。两种检测方法的一致性为97%；因此，选择诊断检测方法是便利性和经验的问题。在所有62例仅提供了一份血样的麻疹病例中，两种方法均检测到了麻疹IgM特异性抗体。62例病例中有50%在出疹后3天内呈阳性。出疹后10天，这一比例增至91%。
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