爱泼斯坦-巴尔病毒的潜伏性DNA：在中性甘油梯度中与高分子量细胞DNA分离
Latent DNA of Epstein-Barr virus: separation from high-molecular-weight cell DNA in a neutral glycerol gradient.
作者信息
Tanaka A, Nonoyama M
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4658-61. doi: 10.1073/pnas.71.12.4658.
Abstract
Epstein-Barr virus DNA in virus non-productive cells was separated from high-molecular-weight cell DNA by sedimentation through a neutral glycerol gradient after gentle lysis of cells by Pronase and Sarkosyl. The isolated Epstein-Barr virus DNA had a density of 1.716-1.717 g/cm(3) in CsCl equilibrium centrifugation, which is very close to the virus DNA density of 1.718 g/cm(3). The results indicated that the majority of Epstein-Barr virus DNA in virus nonproductive cells is not covalently integrated into high-molecular-weight cell DNA.
摘要
在用链霉蛋白酶和十二烷基肌氨酸钠轻柔裂解细胞后，通过中性甘油梯度沉降，将病毒非生产性细胞中的爱泼斯坦-巴尔病毒DNA与高分子量细胞DNA分离。在氯化铯平衡离心法中，分离出的爱泼斯坦-巴尔病毒DNA密度为1.716 - 1.717 g/cm³，这与病毒DNA密度1.718 g/cm³非常接近。结果表明，病毒非生产性细胞中的大多数爱泼斯坦-巴尔病毒DNA并非共价整合到高分子量细胞DNA中。
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