Kim J S, Ishikawa H, Sung K R, Xu J, Wollstein G, Bilonick R A, Gabriele M L, Kagemann L, Duker J S, Fujimoto J G, Schuman J S
UPMC Eye Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, 203 Lothrop Street, Eye and Ear Institute, Suite 834, Pittsburgh, PA 15213, USA.
Br J Ophthalmol. 2009 Aug;93(8):1057-63. doi: 10.1136/bjo.2009.157875. Epub 2009 May 7.
BACKGROUND/AIMS: To investigate retinal nerve fibre layer (RNFL) thickness measurement reproducibility using conventional time-domain optical coherence tomography (TD-OCT) and spectral-domain OCT (SD-OCT), and to evaluate two methods defining the optic nerve head (ONH) centring: Centred Each Time (CET) vs Centred Once (CO), in terms of RNFL thickness measurement variability on SD-OCT.
Twenty-seven eyes (14 healthy subjects) had three circumpapillary scans with TD-OCT and three raster scans (three-dimensional or 3D image data) around ONH with SD-OCT. SD-OCT images were analysed in two ways: (1) CET: ONH centre was defined on each image separately and (2) CO: ONH centre was defined on one image and exported to other images after scan registration. After defining the ONH centre, a 3.4 mm diameter virtual circular OCT was resampled on SD-OCT images to mimic the conventional circumpapillary RNFL thickness measurements taken with TD-OCT.
CET and CO showed statistically significantly better reproducibility than TD-OCT except for 11:00 with CET. CET and CO methods showed similar reproducibility.
SD-OCT 3D cube data generally showed better RNFL measurement reproducibility than TD-OCT. The choice of ONH centring methods did not affect RNFL measurement reproducibility.
背景/目的：使用传统时域光学相干断层扫描（TD-OCT）和频域光学相干断层扫描（SD-OCT）研究视网膜神经纤维层（RNFL）厚度测量的可重复性，并在SD-OCT上评估两种定义视神经乳头（ONH）中心的方法：每次居中（CET）与一次居中（CO），比较其对RNFL厚度测量变异性的影响。
对27只眼（14名健康受试者）进行TD-OCT的三次视乳头周围扫描以及SD-OCT对视神经乳头周围的三次光栅扫描（三维或3D图像数据）。SD-OCT图像通过两种方式进行分析：（1）CET：在每张图像上分别定义ONH中心；（2）CO：在一张图像上定义ONH中心，并在扫描配准后将其导出到其他图像上。定义ONH中心后，在SD-OCT图像上对直径3.4 mm的虚拟圆形OCT进行重采样，以模拟用TD-OCT进行的传统视乳头周围RNFL厚度测量。
除CET在11:00方向外，CET和CO的可重复性在统计学上显著优于TD-OCT。CET和CO方法显示出相似的可重复性。
SD-OCT的3D立方数据通常显示出比TD-OCT更好的RNFL测量可重复性。ONH中心定位方法的选择不影响RNFL测量的可重复性。
