Maran B A Venmathi, Iwamoto Emi, Okuda Jun, Matsuda Shuhei, Taniyama Shigeto, Shida Yasuo, Asakawa Manabu, Ohtsuka Susumu, Nakai Toshihiro, Boxshall Geoffrey A
Takehara Marine Science Station, Setouchi Field Science Center, Graduate School of Biosphere Science, Hiroshima University, 5-8-1 Minato-machi, Takehara 725 0024, Japan.
Toxicon. 2007 Nov;50(6):779-90. doi: 10.1016/j.toxicon.2007.06.021. Epub 2007 Jul 5.
A total of 50 bacterial isolates was obtained from the copepod Pseudocaligus fugu, which is a common parasite, collected from the body surface of the panther puffer Takifugu pardalis. On the basis of colony characteristics, these bacterial isolates were grouped into six types, of which only two (Types-I and -II) showed a high affinity for adhesion to the carapace of the banana shrimp Penaeus merguiensis. These two types of adhesive bacteria were identified through 16S rRNA sequence analysis as Shewanella woodyi (Type-I) and Roseobacter sp. (Type-II). Representative isolates of these two adhesive bacteria were examined for tetrodotoxin (TTX) production by high-performance liquid chromatography (HPLC)-fluorometric system, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). It was rather unexpectedly revealed that TTX and anhydroTTX were present in the supernatant of culture of the Type-II isolate Roseobacter sp.
从豹纹东方鲀（Takifugu pardalis）体表采集的常见寄生虫——日本伪锚盘虫（Pseudocaligus fugu）桡足类动物中，共获得了50株细菌分离株。根据菌落特征，这些细菌分离株被分为六种类型，其中只有两种（I型和II型）对墨吉对虾（Penaeus merguiensis）的头胸甲表现出高粘附亲和力。通过16S rRNA序列分析，这两种粘附细菌被鉴定为伍氏希瓦氏菌（Shewanella woodyi，I型）和玫瑰杆菌属（Roseobacter sp.，II型）。通过高效液相色谱（HPLC）-荧光系统、气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS），对这两种粘附细菌的代表性分离株进行了河豚毒素（TTX）产生情况检测。结果出人意料地发现，II型分离株玫瑰杆菌属的培养上清液中存在TTX和脱水TTX。
