一株来自虫纹东方鲀河豚的产河豚毒素弧菌菌株LM-1。
A tetrodotoxin-producing Vibrio strain, LM-1, from the puffer fish Fugu vermicularis radiatus.
作者信息
Lee M J, Jeong D Y, Kim W S, Kim H D, Kim C H, Park W W, Park Y H, Kim K S, Kim H M, Kim D S
机构信息
Department of Diagnosis, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, Kyung-Ju 780-714, Korea.
出版信息
Appl Environ Microbiol. 2000 Apr;66(4):1698-701. doi: 10.1128/AEM.66.4.1698-1701.2000.
Abstract
Identification of tetrodotoxin (TTX) and its derivatives produced from a Vibrio strain in the intestine of the puffer fish Fugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, high-performance liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated that the isolated bacterium produced TTX, 4-epi-TTX, and anhTTX during cultivation, suggesting that Vibrio strains are responsible for the toxification of the puffer fish.
摘要
通过薄层色谱法、电泳法、高效液相色谱法和气相色谱-质谱联用技术，结合小鼠毒性生物测定法，对条纹东方鲀肠道中一株弧菌产生的河豚毒素（TTX）及其衍生物进行了鉴定。结果表明，分离出的细菌在培养过程中产生了TTX、4-表TTX和anhTTX，这表明弧菌菌株是导致河豚鱼中毒的原因。
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