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人及牛牙釉质体外诱导侵蚀性病变的进展及表面超微结构

Progression and surface ultrastructure of in vitro caused erosive lesions in human and bovine enamel.

作者信息

Meurman J H, Frank R M

机构信息

Faculté de Chirurgie Dentaire, Université Louis-Pasteur, Strasbourg, France.

出版信息

Caries Res. 1991;25(2):81-7. doi: 10.1159/000261348.

DOI:10.1159/000261348
PMID:2059980
Abstract

Surface prismatic and aprismatic human enamel and unpolished or diamond-polished specimens of bovine enamel, with test and control surfaces on each studied block, were immersed in phosphoric acid containing cola beverage (pH 2.6) and citric or malic acid containing sports drinks (pH 2.8 and 3.4, respectively) for 15-180 min. The specimens were then prepared and studied in a JEOL 35C scanning electron microscope. In human aprismatic surface enamel an irregular type of dissolution was observed, with severely affected areas adjacent to less affected ones. Prismatic human and bovine specimens showed a characteristic dissolution where initial erosion after 15 min immersion was seen to affect specifically the prism sheath areas. Longer immersion caused dissolution of enamel prism cores followed by interprismatic areas. In bovine enamel malic acid affected least the surface ultrastructure when compared with citric and phosphoric acids after 15-30 min of immersion, but thereafter no difference was observed between the acids in causing erosion. Diamond-polished enamel was found more liable to erosion than non-polished enamel. Thus, the structure of enamel greatly modified the progression of in vitro caused erosion, in particular in human tooth specimens.

摘要

将具有棱柱形和非棱柱形表面的人牙釉质以及未经抛光或经金刚石抛光的牛牙釉质标本(每个研究块上都有测试和对照表面)浸入含磷酸的可乐饮料(pH 2.6)以及含柠檬酸或苹果酸的运动饮料(pH分别为2.8和3.4)中15至180分钟。然后制备这些标本并在JEOL 35C扫描电子显微镜下进行研究。在人非棱柱形表面牙釉质中观察到一种不规则的溶解类型,受影响严重的区域与受影响较小的区域相邻。棱柱形的人牙和牛牙标本显示出一种特征性的溶解,在浸泡15分钟后最初的侵蚀特别影响棱柱鞘区域。浸泡时间延长会导致牙釉质棱柱核心随后是柱间质区域的溶解。在浸泡15至30分钟后,与柠檬酸和磷酸相比,苹果酸对牛牙釉质表面超微结构的影响最小,但此后在导致侵蚀方面这些酸之间没有观察到差异。发现经金刚石抛光的牙釉质比未经抛光的牙釉质更容易受到侵蚀。因此,牙釉质的结构极大地改变了体外引起的侵蚀进程,特别是在人牙标本中。

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