Fakhar Mahdi, Chabra Aroona, Rahimi-Esboei Bahman, Rezaei Fatemeh
Molecular and Cell Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Student Research Committee, School of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
J Parasit Dis. 2015 Jun;39(2):162-7. doi: 10.1007/s12639-013-0300-y. Epub 2013 May 15.
Hydatidosis is caused by a tapeworm which infects humans by the larval stage. In humans, the disease is so serious that it requires surgery for treatment. Documents show that there have been many efforts in finding new scolicidal agents for reducing the rate of the infection. The objective of this study was determination of the scolicidal effect of two fungal chitosan types, produced from Penicillium spp. and commercially chitosan (CC) on Echinococcus granulosus protoscolex. Protoscolices were aseptically aspirated from sheep livers hydatid cysts. Four concentrations (50, 100, 200, 400 μg/ml) of each type of prepared chitosan were used for 10, 30, 60 and 180 min. Viability of protoscolices was detected by 0.1 % eosin staining. Fungal chitosan which was the most bioactive type with higher degree of deacetylation showed stronger scolicidal activity in vitro (P < 0.05). Fungal chitosan could be recommended, as good as CC for hydatid cysts control and is a noble alternative for synthetic and chemical scolicidal.
包虫病由一种绦虫引起，该绦虫通过幼虫阶段感染人类。在人类中，这种疾病非常严重，需要通过手术进行治疗。文献表明，人们为寻找新的杀头节剂以降低感染率付出了诸多努力。本研究的目的是测定由青霉属真菌产生的两种壳聚糖以及市售壳聚糖（CC）对细粒棘球绦虫原头节的杀头节效果。原头节从绵羊肝脏包虫囊肿中无菌吸出。每种制备的壳聚糖的四种浓度（50、100、200、400μg/ml）分别作用10、30、60和180分钟。通过0.1%伊红染色检测原头节的活力。脱乙酰度较高、生物活性最强的真菌壳聚糖在体外表现出更强的杀头节活性（P<0.05）。真菌壳聚糖在控制包虫囊肿方面与CC一样有效，可被推荐作为合成和化学杀头节剂的理想替代品。
