用于研究胚胎发育的合成策略。
Synthetic strategies for studying embryonic development.
作者信息
Ouyang Xiaohu, Chen James K
机构信息
Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
出版信息
Chem Biol. 2010 Jun 25;17(6):590-606. doi: 10.1016/j.chembiol.2010.04.013.
Abstract
Developmental biology has evolved from a descriptive science to one based on genetic principles and molecular mechanisms. Although molecular biology and genetic technologies have been the primary drivers of this transformation, synthetic strategies have been increasingly utilized to interrogate the mechanisms of embryonic patterning with spatial and temporal precision. In this review, we survey how chemical tools and engineered proteins have been used to perturb developmental processes at the DNA, RNA, protein, and cellular levels. We discuss the design principles, experimental capabilities, and limitations of each method, as well as future challenges for the chemical and developmental biology communities.
摘要
发育生物学已从一门描述性科学发展成为一门基于遗传原理和分子机制的学科。尽管分子生物学和基因技术一直是这一转变的主要驱动力，但合成策略已越来越多地被用于精确地从空间和时间上探究胚胎模式形成的机制。在本综述中，我们概述了化学工具和工程蛋白如何被用于在DNA、RNA、蛋白质和细胞水平上干扰发育过程。我们讨论了每种方法的设计原则、实验能力和局限性，以及化学和发育生物学界未来面临的挑战。
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