Centro Fecondazione Assistita, Clinica Villa del Sole, Napoli, Italy.
J Assist Reprod Genet. 2010 Sep;27(9-10):549-54. doi: 10.1007/s10815-010-9452-1. Epub 2010 Jul 17.
To compare embryo survival, pregnancy and implantation rates after cryopreservation of human cleavage-stage embryos with slow-rate cryopreservation or vitrification.
262 patients, attending for assisted reproduction, were prepared for oocyte retrieval using standard controlled ovarian hyperstimulation protocols. Excess embryos were cryopreserved on day 3 either by vitrification, or slow-rate cryopreservation in a programmable freezer. Cycles of thawing were monitored for thaw efficiency, pregnancy and implantation rates.
Clinical pregnancy and implantation rates were highly comparable between cycles in which day 3 embryos were thawed either after slow-rate cryopreservation or vitrification.
These data suggest that vitrification of human embryos during assisted reproduction cycles achieves comparable success rates to fresh cycles and therefore can be applied in the laboratory of assisted reproduction.
比较慢速冷冻法和玻璃化冷冻法对人卵裂期胚胎进行冷冻保存后胚胎存活率、妊娠率和种植率。
262 名接受辅助生殖的患者，采用标准的控制性卵巢超排卵方案准备取卵。第 3 天，多余的胚胎通过玻璃化冷冻法或程序冷冻仪的慢速冷冻法进行冷冻保存。监测解冻周期的解冻效率、妊娠率和种植率。
慢速冷冻法和玻璃化冷冻法解冻第 3 天胚胎的周期之间，临床妊娠率和种植率高度相似。
这些数据表明，辅助生殖周期中人类胚胎的玻璃化冷冻与新鲜周期的成功率相当，因此可以在辅助生殖实验室中应用。
