使用新型冷冻保存设备（塑料薄片）进行玻璃化冷冻人胚胎移植的临床结局。
Clinical outcomes resulting from the transfer of vitrified human embryos using a new device for cryopreservation (plastic blade).
机构信息
Division of Reproductive Medicine, Sugiyama Clinic, 1-53-1, Ohara, Setagaya, Tokyo, 156-0041, Japan.
出版信息
J Assist Reprod Genet. 2010 Apr;27(4):161-7. doi: 10.1007/s10815-010-9390-y. Epub 2010 Feb 2.
PURPOSE
We attempted clinical application of a plastic blade, which is a novel cryopreservation device, for vitrification of human embryos and blastocysts.
METHODS
Between February 2003 and December 2007, a total of 4,430 Day 3 embryos from 898 patients (Day 3 group) and 55 blastocysts from 29 patients (blastocyst group) were vitrified and cryopreserved with a plastic device, and subsequently thawed for embryo transfer. Clinical outcomes after thawing and transfer of vitrified embryos and blastocysts were evaluated.
RESULTS
In the Day 3 group, all embryos resulting from 1,441 oocyte retrieval cycles were recovered, and the thawed embryo survival rate was 98.4%. In the blastocyst group, the survival rate after thawing was 100%. A total of 3,026 day 3 embryos and 46 blastocysts were transferred. The pregnancy and implantation rates in the Day 3 group were 25.0% and 15.5%, respectively, and in the blastocyst group the rates were 24.2% and 26.1%, respectively. The miscarriage rates in the Day 3 and blastocyst groups were 18.3% and 50.0%, respectively.
CONCLUSIONS
A plastic blade is a useful novel device in cryopreservation of vitrified human embryos.
目的
我们尝试将一种新型的冷冻保存装置——塑料刀片应用于人类胚胎和囊胚的玻璃化冷冻。
方法
在 2003 年 2 月至 2007 年 12 月期间，我们使用塑料装置对 898 名患者的 4430 枚第三天胚胎（第三天组）和 29 名患者的 55 枚囊胚（囊胚组）进行了玻璃化冷冻和保存，随后进行解冻以进行胚胎移植。评估了解冻和移植冷冻胚胎和囊胚后的临床结局。
结果
在第三天组中，我们回收了 1441 个卵母细胞回收周期的所有胚胎，解冻后的胚胎存活率为 98.4%。在囊胚组中，解冻后的存活率为 100%。共移植了 3026 枚第三天胚胎和 46 枚囊胚。第三天组的妊娠和着床率分别为 25.0%和 15.5%，囊胚组的妊娠和着床率分别为 24.2%和 26.1%。第三天组和囊胚组的流产率分别为 18.3%和 50.0%。
结论
塑料刀片是一种用于人类胚胎玻璃化冷冻保存的有用的新型装置。
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