McNulty M S, Curran W L, Allan G M, McFerran J B
Arch Virol. 1978;58(3):193-202. doi: 10.1007/BF01317601.
PK-15 cells infected with pig and lamb rotavirus strains which were not adapted to serial growth in cell cultures were examined by electron microscopy. A major difference between virus morphogenesis in the initial passage in PK-15 cells and in intestinal epithelial cells was the generation of large numbers of coreless virus particles in PK-15 cells. The numbers of coreless particles increased with increasing multiplicity of infection. Infectious virus was synthesized in PK-15 cells, but a variable decrease in infectivity titre occurred between 12 and 24 hours after infection. It is suggested that synthesis of defective interfering particles or an inhibitory substance such as interferon might account for this decrease.
用电子显微镜检查了感染猪和羊轮状病毒株但未适应在细胞培养中连续传代生长的PK-15细胞。PK-15细胞初次传代时病毒形态发生与肠道上皮细胞中的主要区别在于PK-15细胞中产生了大量无核心病毒颗粒。无核心颗粒的数量随感染复数的增加而增加。PK-15细胞中合成了传染性病毒，但感染后12至24小时之间感染性滴度出现了不同程度的下降。推测缺陷干扰颗粒或干扰素等抑制性物质的合成可能是导致这种下降的原因。
