从奇异柠檬酸杆菌的R因子中克隆和测序编码氨基糖苷6'-N-乙酰基转移酶的基因。
Cloning and sequencing of a gene encoding an aminoglycoside 6'-N-acetyltransferase from an R factor of Citrobacter diversus.
作者信息
Tenover F C, Filpula D, Phillips K L, Plorde J J
机构信息
Seattle Veterans Administration Medical Center, Washington 98108.
出版信息
J Bacteriol. 1988 Jan;170(1):471-3. doi: 10.1128/jb.170.1.471-473.1988.
Abstract
The aacA1 gene, which encodes a 6'-N-acetyltransferase [AAC(6')-I] mediating resistance to kanamycin, tobramycin, and amikacin, was cloned from the Citrobacter diversus R plasmid pBWH100 into the Escherichia coli vector pBR322. The complete nucleotide sequence of the gene and flanking regions was determined. A protein of approximately 21 kilodaltons was identified when the chimeric plasmid encoding the aacA1 gene was introduced into E. coli maxicells. This value is consistent with the size predicted for a protein translated from the open reading frame of the gene.
摘要
编码介导对卡那霉素、妥布霉素和阿米卡星耐药的6'-N-乙酰基转移酶【AAC(6')-I】的aacA1基因，从奇异柠檬酸杆菌R质粒pBWH100克隆至大肠杆菌载体pBR322。测定了该基因及其侧翼区域的完整核苷酸序列。当将编码aacA1基因的嵌合质粒导入大肠杆菌最大细胞时，鉴定出一种约21千道尔顿的蛋白质。该值与从该基因的开放阅读框翻译的蛋白质预测大小一致。
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