Hannecart-Pokorni E, Depuydt F, de wit L, van Bossuyt E, Content J, Vanhoof R
Pasteur Institute-Brussels, Belgium.
Antimicrob Agents Chemother. 1997 Feb;41(2):314-8. doi: 10.1128/AAC.41.2.314.
The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.
对来自弗氏柠檬酸杆菌Cf155的编码氨基糖苷6'-N-乙酰基转移酶的阿米卡星抗性基因aac(6')-Im[校正后]进行了表征。该基因被鉴定为一个521 bp的编码序列，位于整合子5'保守区段的下游。这个aac(6')-Im[校正后]基因的序列对应于一个173个氨基酸的蛋白质，该蛋白质在165个氨基酸的重叠区域与aac(6')-Ia基因产物具有64.2%的同一性（F.C. Tenover、D. Filpula、K.L. Phillips和J.J. Plorde，《细菌学杂志》170:471-473，1988年）。通过使用聚合酶链反应（PCR），在来自比利时两家医院的86株革兰氏阴性临床分离株中的8株中检测到了aac(6')-Im[校正后]基因，包括柠檬酸杆菌、克雷伯菌属和大肠杆菌的分离株。对这些分离株中aac(6')-Im[校正后]基因环境的PCR定位表明，该基因位于一个sulI型整合子内；插入区域长1700个碱基，包括两个基因盒，第二个是ant(3")-Ib。
