Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Int J Hyperthermia. 2010;26(6):577-85. doi: 10.3109/02656736.2010.485594.
To carry out a preliminary study examining the efficacy of long-term hot-tub therapy (HTT) in the improvement of diabetic complications on streptozotocin-induced diabetic rats.
Male Wistar rats were immersed mid-sternum in a circulating water bath (42 degrees C for 30 min) to obtain a core body temperature of 41 degrees C; this process was repeated three times a week for 5 months. The blood was collected every month. Multiple parameters were examined for all rats including heat shock protein (Hsp70) level, serum glucose and insulin concentrations, advanced glycation end product (AGE) and glycated haemoglobin (HbA1c) formation, lipid profile and antioxidant defence system. Additionally, the chaperoning capacity of glycated Hsp70 was evaluated based on in vitro studies in which the refolding of denatured luciferase was compared to refolding by native Hsp70.
HTT-treated diabetic rats showed a significant improvement in lipid profile, antioxidant capacity, insulin secretion and serum Hsp70 level and a significant decrease in AGE formation compared to the untreated diabetic rats. However, HTT had a borderline significant effect on weight and fasting blood glucose. Glycated Hsp70 lost its chaperoning ability to reactivate the denatured luciferase.
A decrease in complications in diabetic rats after hot-tub therapy is shown here. An increase in the extracellular Hsp70 level due to HTT was observed. This increase may serve to protect the structure of proteins (e.g. preventing AGE formation), and the observed beneficial effects may be related to it.
初步研究长期热水浴疗法（HTT）对链脲佐菌素诱导的糖尿病大鼠糖尿病并发症改善的疗效。
雄性 Wistar 大鼠浸入循环水浴（42°C 30 分钟）以获得 41°C 的核心体温；每周重复此过程三次，共进行 5 个月。每月采集血液。对所有大鼠进行包括热休克蛋白（Hsp70）水平、血清葡萄糖和胰岛素浓度、晚期糖基化终产物（AGE）和糖化血红蛋白（HbA1c）形成、血脂谱和抗氧化防御系统在内的多项参数检测。此外，还评估了糖化 Hsp70 的伴侣能力，其方法是将变性荧光素酶的重折叠与天然 Hsp70 的重折叠进行比较。
与未经治疗的糖尿病大鼠相比，HTT 治疗的糖尿病大鼠的血脂谱、抗氧化能力、胰岛素分泌和血清 Hsp70 水平显著改善，AGE 形成显著减少。然而，HTT 对体重和空腹血糖的影响具有边缘显著性。糖化 Hsp70 丧失了重新激活变性荧光素酶的伴侣能力。
本研究显示，热水浴治疗后糖尿病大鼠的并发症减少。由于 HTT，细胞外 Hsp70 水平增加。这种增加可能有助于保护蛋白质的结构（例如，防止 AGE 形成），并且观察到的有益效果可能与之相关。
