Shieh M T, Pierce C, Bartholomew W, Kumar V
Department of Medical Technology, University at Buffalo, SUNY 14214.
Immunol Invest. 1990 Oct-Dec;19(5-6):507-18. doi: 10.3109/08820139009052976.
Controversies exist as to the differences in the specificities of phospholipid antibodies in SLE and syphilis. We report an ELISA assay that could distinguish phospholipid antibodies associated with SLE and syphilis based on their differential reactions with phosphatidyl choline and VDRL antigens. Antibodies to phospholipid from patients with SLE reacted equally well when tested with these two antigens in the ELISA assay whereas phospholipid antibodies present in syphilis patients exhibited little or no binding to phosphatidyl choline. There were no differences in the binding of phospholipid antibodies to other phospholipids such as cardiolipin and phosphatidyl serine. In addition, there was no association of anti-phospholipid antibodies with the presence of either DNA or RPR antibodies suggesting their distinctness from each other.
关于系统性红斑狼疮（SLE）和梅毒中磷脂抗体特异性的差异存在争议。我们报告了一种酶联免疫吸附测定（ELISA）方法，该方法可以根据磷脂抗体与磷脂酰胆碱和性病研究实验室玻片试验（VDRL）抗原的不同反应来区分与SLE和梅毒相关的磷脂抗体。在ELISA试验中，用这两种抗原检测时，SLE患者的磷脂抗体反应同样良好，而梅毒患者体内存在的磷脂抗体与磷脂酰胆碱几乎没有或没有结合。磷脂抗体与其他磷脂如心磷脂和磷脂酰丝氨酸的结合没有差异。此外，抗磷脂抗体与DNA或快速血浆反应素环状卡片试验（RPR）抗体的存在无关，表明它们彼此不同。
