Sreejith G, Latha P G, Shine V J, Anuja G I, Suja S R, Sini S, Shyama S, Pradeep S, Shikha P, Rajasekharan S
Malankara Catholic College, Mariagiri, Kaliakkavilai 629 153, India.
Indian J Exp Biol. 2010 May;48(5):494-8.
Cassia occidentalis Linn. mast cell degranulation at a dose of 250 mg/kg, showed dose dependent stabilizing activity towards human RBC, with is widely used in traditional medicine of India to treat a number of clinical conditions including allergy and inflammatory manifestations. In the present study anti-allergic, anti-inflammatory and anti-oxidant properties of C. occidentalis whole plant ethanolic extract (CO) was investigated. Effects of CO on rat mast cell degranulation inhibition and human red blood cell (HRBC) membrane stabilization were studied in vitro following standard methods. The anti lipidperoxidant effects of CO were also studied in vitro. Effect of CO on carrageenan-induced mouse paw oedema inhibition was also assessed. CO significantly decreased maximum protection of 80.8% at 15 microg/ml. The extract also caused significant reduction in malondialdehyde (MDA) levels of murine hepatic microsomes at 100 microg/ml (56%) and significantly reduced carrageenan induced inflammation in mice at a dose of 250 mg/kg. Results of the present study indicated that CO inhibited mast cell degranulation, stabilized HRBC membrane thereby alleviating immediate hypersensitivity besides showing anti oxidant activity.
决明子在剂量为250毫克/千克时可使肥大细胞脱颗粒，对人红细胞显示出剂量依赖性的稳定活性，在印度传统医学中被广泛用于治疗多种临床病症，包括过敏和炎症表现。在本研究中，对决明子全株乙醇提取物（CO）的抗过敏、抗炎和抗氧化特性进行了研究。按照标准方法在体外研究了CO对大鼠肥大细胞脱颗粒抑制和人红细胞（HRBC）膜稳定的作用。还在体外研究了CO的抗脂质过氧化作用。评估了CO对角叉菜胶诱导的小鼠爪肿胀抑制的作用。CO在15微克/毫升时显著降低了80.8%的最大保护率。该提取物在100微克/毫升时还使小鼠肝微粒体的丙二醛（MDA）水平显著降低（56%），并在剂量为250毫克/千克时显著减轻了角叉菜胶诱导的小鼠炎症。本研究结果表明，CO抑制肥大细胞脱颗粒，稳定HRBC膜，从而除了显示抗氧化活性外还减轻速发型超敏反应。
