TCT 基序，翻译机器中转录系统中 RNA 聚合酶 II 的关键组件。
The TCT motif, a key component of an RNA polymerase II transcription system for the translational machinery.
机构信息
Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA.
出版信息
Genes Dev. 2010 Sep 15;24(18):2013-8. doi: 10.1101/gad.1951110. Epub 2010 Aug 27.
Abstract
The TCT motif (polypyrimidine initiator) encompasses the transcription start site of nearly all ribosomal protein genes in Drosophila and mammals. The TCT motif is required for transcription of ribosomal protein gene promoters. The TCT element resembles the Inr (initiator), but is not recognized by TFIID and cannot function in lieu of an Inr. However, a single T-to-A substitution converts the TCT element into a functionally active Inr. Thus, the TCT motif is a novel transcriptional element that is distinct from the Inr. These findings reveal a specialized TCT-based transcription system that is directed toward the synthesis of ribosomal proteins.
摘要
TCT 基序（多嘧啶起始子）包含了果蝇和哺乳动物中几乎所有核糖体蛋白基因的转录起始位点。TCT 基序是核糖体蛋白基因启动子转录所必需的。TCT 元件类似于 Inr（起始子），但不被 TFIID 识别，不能替代 Inr 发挥作用。然而，单个 T 到 A 的替换将 TCT 元件转化为具有功能活性的 Inr。因此，TCT 基序是一种与 Inr 不同的新型转录元件。这些发现揭示了一种专门针对核糖体蛋白合成的基于 TCT 的转录系统。
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本文引用的文献
1
A paired-end sequencing strategy to map the complex landscape of transcription initiation.一种用于绘制转录起始复杂景观的配对末端测序策略。
Nat Methods. 2010 Jul;7(7):521-7. doi: 10.1038/nmeth.1464. Epub 2010 May 23.
2
Three key subregions contribute to the function of the downstream RNA polymerase II core promoter.三个关键亚区有助于下游 RNA 聚合酶 II 核心启动子的功能。
Mol Cell Biol. 2010 Jul;30(14):3471-9. doi: 10.1128/MCB.00053-10. Epub 2010 May 10.
3
Shifting players and paradigms in cell-specific transcription.细胞特异性转录中的角色转换和范式转变。
Mol Cell. 2009 Dec 25;36(6):924-31. doi: 10.1016/j.molcel.2009.12.011.
4
Promoting developmental transcription.促进发育转录。
Development. 2010 Jan;137(1):15-26. doi: 10.1242/dev.035493.
5
Regulation of gene expression via the core promoter and the basal transcriptional machinery.通过核心启动子和基础转录机制调控基因表达。
Dev Biol. 2010 Mar 15;339(2):225-9. doi: 10.1016/j.ydbio.2009.08.009. Epub 2009 Aug 13.
6
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7
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Mol Cell. 2008 Oct 10;32(1):96-105. doi: 10.1016/j.molcel.2008.09.009.
8
MachiBase: a Drosophila melanogaster 5'-end mRNA transcription database.MachiBase：一个黑腹果蝇5'端mRNA转录数据库。
Nucleic Acids Res. 2009 Jan;37(Database issue):D49-53. doi: 10.1093/nar/gkn694. Epub 2008 Oct 8.
9
TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.TBP、Mot1和NC2建立了一个调控回路，该回路控制依赖DPE与依赖TATA的转录。
Genes Dev. 2008 Sep 1;22(17):2353-8. doi: 10.1101/gad.1681808. Epub 2008 Aug 14.
10
Switching of the core transcription machinery during myogenesis.成肌过程中核心转录机制的转换。
Genes Dev. 2007 Sep 1;21(17):2137-49. doi: 10.1101/gad.1583407. Epub 2007 Aug 17.
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