Department of Pharmacology and Therapeutics, McGill University, Montreal, Canada H3G 1Y6.
Microsc Res Tech. 2011 Jul;74(7):592-604. doi: 10.1002/jemt.20928. Epub 2010 Sep 9.
Nanoparticles are the latest tool acquired by the science of bioimaging, serving primarily as new contrast agents, sensors, or signal enhancing agents in established and developing imaging techniques. This review focuses on the unique properties of two classes of nanoparticles: gold nanoparticles (GNP) and quantum dots, and how these properties are benefiting cellular and in vivo imaging. We discuss the surface plasmon resonance of GNP and its implications for various imaging techniques of biological relevance. Furthermore, the key properties of quantum dots are reviewed, and their use alone or in combination with traditional fluorescent dyes for biological imaging are described. The underlying principles of these techniques are provided, along with some representative examples.
纳米粒子是生物成像科学最新采用的工具,主要用作现有和新兴成像技术中的新型对比剂、传感器或信号增强剂。本综述重点介绍两类纳米粒子的独特性质:金纳米粒子(GNP)和量子点,以及这些性质如何使细胞和体内成像受益。我们讨论了 GNP 的表面等离子体共振及其对各种具有生物学意义的成像技术的影响。此外,还回顾了量子点的关键性质,并描述了它们单独使用或与传统荧光染料结合用于生物成像的情况。提供了这些技术的基本原理,并给出了一些代表性的例子。