Proteogenomics Research Institute for Systems Medicine, San Diego, CA, USA.
Int J Nanomedicine. 2010 Sep 7;5:653-9. doi: 10.2147/IJN.S11677.
Silver nanoparticles are increasingly finding applications in medicine; however, little is known about their in vivo tissue distribution. Here, we have developed a rapid method for radiolabeling of silver nanoparticles with iodine-125 in order to track in vivo tissue uptake of silver nanoparticles after systemic administration by biodistribution analysis and single-photon emission computerized tomography (SPECT) imaging. Poly(N-vinyl-2 -pyrrolidone)-capped silver nanoparticles with an average size of 12 nm were labeled by chemisorption of iodine-125 with a > 80% yield of radiolabeling efficiency. Radiolabeled silver nanoparticles were intravenously injected in Balb/c mice, and the in vivo distribution pattern of these nanoparticles was evaluated by noninvasive whole-body SPECT imaging, which revealed uptake of the nanoparticles in the liver and spleen. Biodistribution analysis confirmed predominant accumulation of the silver nanoparticles in the spleen (41.5%ID/g) and liver (24.5%ID/g) at 24 h. Extensive uptake in the tissues of the reticuloendothelial system suggests that further investigation of silver nanoparticle interaction with hepatic and splenic tissues at the cellular level is critical for evaluation of the in vivo effects and potential toxicity of silver nanoparticles. This method enables rapid iodine-125 radiolabeling of silver nanoparticles with a specific activity sufficient for in vivo imaging and biodistribution analysis.
银纳米粒子在医学中的应用越来越广泛；然而，人们对其体内组织分布知之甚少。在这里，我们开发了一种快速的方法，用碘-125 标记银纳米粒子，以便通过生物分布分析和单光子发射计算机断层扫描（SPECT）成像来跟踪全身给药后银纳米粒子在体内的组织摄取。用碘-125 化学吸附对平均粒径为 12nm 的聚（N-乙烯基-2-吡咯烷酮）封端的银纳米粒子进行标记，标记效率>80%。放射性标记的银纳米粒子通过静脉注射到 Balb/c 小鼠体内，通过非侵入性全身 SPECT 成像评估这些纳米粒子的体内分布模式，结果显示纳米粒子在肝脏和脾脏中被摄取。生物分布分析证实，24 小时后，银纳米粒子主要在脾脏（41.5%ID/g）和肝脏（24.5%ID/g）中积累。在网状内皮系统的组织中大量摄取表明，进一步研究银纳米粒子与肝和脾组织的细胞水平相互作用对于评估银纳米粒子的体内效应和潜在毒性至关重要。该方法能够快速用碘-125 对银纳米粒子进行放射性标记，其比活度足以进行体内成像和生物分布分析。
