Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China.
Virol Sin. 2010 Apr;25(2):98-106. doi: 10.1007/s12250-010-3102-8. Epub 2010 Apr 9.
The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.
对 EV71 流行株的生物学特性和遗传背景进行比较分析，被普遍认为是开发有效 EV71 疫苗的必要基础工作。在本研究中，我们对从中国阜阳、合肥、昆明和深圳分离到的 5 株 EV71 流行株进行了测序，分别命名为 FY-23、FY-22、H44、K9 和 S1。序列比对表明它们的基因型为 C4。这些分离株的 VP1 基因的遗传距离表明，它们与中国先前报道的其他 EV71 株具有高度的同源性，遗传同一性相似。此外，这些分离株在 Vero 细胞中增殖时表现出一些明显的增殖动力学和蚀斑形态。然而，在新生小鼠中的致病性能力方面存在明显的差异。在交叉中和试验和免疫原性分析中也发现了一些差异。所有这些结果都与中国流行的 EV71 株的生物学特性有关，并有助于未来开发 EV71 疫苗。
