Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas, USA.
Cardiovasc Diabetol. 2010 Oct 29;9:71. doi: 10.1186/1475-2840-9-71.
Baboons (Papio hamadryas Sp.) develop features of the cardiometabolic syndrome and represent a clinically-relevant animal model in which to study the aetiology of the disorder. To further evaluate the baboon as a model for the study of the cardiometabolic syndrome, we developed a high sugar high fat diet and hypothesized that it could be used to induce adiposity gain and affect associated circulating biomarkers.
We developed a diet enriched with monosaccharides and saturated fatty acids that was composed of solid and liquid energy sources. We provided a group of baboons (n = 9) ad libitum access to this diet for 8 weeks. Concurrently, a control group (n = 6) was maintained with ad libitum access to a low sugar low fat baseline diet and normal water for 8 weeks. Body composition was determined by dual-energy X-ray absorptiometry and circulating metabolic biomarkers were measured using standard methodology before and after the 8 week study period.
Neither body composition nor circulating biomarkers changed in the control group. Following the 8 weeks, the intervention group had a significant increase in fat mass (1.71 ± 0.98 vs. 3.23 ± 1.70 kg, p = 0.004), triglyceride (55 ± 13 vs. 109 ± 67 mg/dL, p = 0.006,), and leptin (1.19 ± 1.40 vs. 3.29 ± 2.32 ng/mL, p = 0.001) and a decline in adiponectin concentrations (33530 ± 9744 vs. 23330 ± 7863 ng/mL, p = 0.002). Percentage haemoglobin A1C (4.0 ± 0.3 vs. 6.0 ± 1.4, p = 0.002) also increased in the intervention group.
Our findings indicate that when exposed to a high sugar high fat diet, young adult male baboons develop increased body fat and triglyceride concentrations, altered adipokine concentrations, and evidence of altered glucose metabolism. Our findings are in keeping with observations in humans and further demonstrate the potential utility of this highly clinically-relevant animal model for studying diet-induced metabolic dysregulation.
狒狒（Papio hamadryas Sp.）具有代谢综合征的特征，是一种具有临床相关性的动物模型，可用于研究该疾病的病因。为了进一步评估狒狒作为代谢综合征研究模型的价值，我们开发了一种富含单糖和饱和脂肪酸的高糖高脂肪饮食，并假设该饮食可用于诱导肥胖和影响相关循环生物标志物。
我们开发了一种富含单糖和饱和脂肪酸的饮食，由固体和液体能量源组成。我们让一组狒狒（n=9）随意进食这种饮食 8 周。同时，对照组（n=6）在 8 周的研究期间，以随意进食低糖低脂肪的基础饮食和正常水的方式维持。在研究前后，使用标准方法通过双能 X 射线吸收法确定身体成分，使用标准方法测量循环代谢生物标志物。
对照组的身体成分或循环生物标志物均未发生变化。8 周后，干预组的脂肪量显著增加（1.71±0.98 与 3.23±1.70kg，p=0.004），甘油三酯（55±13 与 109±67mg/dL，p=0.006）和瘦素（1.19±1.40 与 3.29±2.32ng/mL，p=0.001）增加，而脂联素浓度降低（33530±9744 与 23330±7863ng/mL，p=0.002）。干预组的血红蛋白 A1C 百分比（4.0±0.3 与 6.0±1.4，p=0.002）也增加。
我们的发现表明，当年轻雄性狒狒接触高糖高脂肪饮食时，会导致体脂肪和甘油三酯浓度增加、脂肪因子浓度改变以及葡萄糖代谢改变的证据。我们的发现与人类的观察结果一致，进一步证明了这种具有高度临床相关性的动物模型在研究饮食引起的代谢失调方面的潜在应用价值。
