Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2353-2357. doi: 10.1099/ijs.0.026211-0. Epub 2010 Oct 29.
A novel, Gram-reaction-positive, aerobic, rod-shaped, non-motile bacterial strain, designated NAL101(T), was isolated from gajami-sikhae, a traditional Korean fermented seafood made of flatfish. Growth occurred at 4-45 °C, at pH 5-10 and in 0-12 % (w/v) NaCl. Optimum growth occurred at 30-37 °C, at pH 8 and in 0-1 % (w/v) NaCl. The cell-wall amino acids were 2,4-diaminobutyric acid, alanine, glycine, threonine and glutamic acid and the major fatty acids were anteiso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0). The predominant menaquinone was MK-11. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The 16S rRNA gene sequence of strain NAL101(T) showed 97.7 % similarity to that of Leucobacter chironomi MM2LB(T), its closest relative. The DNA G+C content was 68.8 mol% and DNA-DNA hybridization values with closely related strains were <22 %. Phylogenetic analyses based on 16S rRNA gene sequences as well as differences in its physiological and biochemical characteristics indicated that strain NAL101(T) represents a novel species of the genus Leucobacter in the family Microbacteriaceae, for which the name Leucobacter celer sp. nov. is proposed. The type strain is NAL101(T) ( = KACC 14220(T) = JCM 16465(T)).
一株新型革兰氏阳性、需氧、杆状、非运动细菌菌株，命名为NAL101(T)，从韩国传统发酵海鲜 gajami-sikhae 中分离得到。该菌在 4-45°C、pH5-10 和 0-12%（w/v）NaCl 条件下生长。最佳生长条件为 30-37°C、pH8 和 0-1%（w/v）NaCl。细胞壁氨基酸组成为 2,4-二氨基丁酸、丙氨酸、甘氨酸、苏氨酸和谷氨酸，主要脂肪酸为anteiso-C(15：0)、iso-C(16：0)和anteiso-C(17：0)。主要的menaquinone 为 MK-11。主要的极性脂为双磷脂酰甘油、磷脂酰甘油和一种未知的糖脂。菌株 NAL101(T)的 16S rRNA 基因序列与最接近的 Leucobacter chironomi MM2LB(T)相似度为 97.7%。DNA G+C 含量为 68.8 mol%，与密切相关菌株的 DNA-DNA 杂交值<22%。基于 16S rRNA 基因序列的系统发育分析以及其生理生化特性的差异表明，菌株 NAL101(T)代表微杆菌科 Leucobacter 属的一个新型物种，建议将其命名为 Leucobacter celer sp. nov.。模式菌株为 NAL101(T)（=KACC 14220(T)=JCM 16465(T)）。
