Institut für Biologie II, Mikrobiologie, Universität Freiburg, 79104 Freiburg, Germany.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):956-960. doi: 10.1099/ijs.0.022780-0. Epub 2010 May 28.
A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31(T), was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus Leucobacter. Binary 16S rRNA gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97 % with Leucobacter alluvii (GenBank accession no: AM072820; 99.4 %), Leucobacter iarius (AM040493; 98.2 %), Leucobacter aridicollis (AJ781047; 97.8 %), Leucobacter komagatae (AB007419; 97.4 %), Leucobacter chironomi (EU346911; 97.1 %) and Leucobacter luti (AM072819; 97.1 %). In contrast, DNA-DNA hybridization experiments showed similarity values below 28 % for DNA samples from the most closely related type strains of L. alluvii, L. aridicollis and L. iarius. Protein analysis by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and automated RiboPrinting using the restriction enzyme PvuII differentiated strain JG 31(T) from all type strains of the genus Leucobacter. The dominant fatty acids of the novel isolate were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0), while the quinone system consisted of menaquinones MK-11, MK-10, MK-9 and MK-8. In a B-type cross-linked peptidoglycan, the cell-wall amino acids were alanine, glycine, threonine, glutamic acid and 2,4-diaminobutyric acid. Strain JG 31(T) was able to grow in a medium containing up to 300 mM K(2)CrO(4) and showed cellular aggregation in response to chromate stress. From biochemical and genomic analyses, the new strain is considered to represent a novel species of the genus Leucobacter, for which the name Leucobacter chromiiresistens sp. nov. is proposed. The type strain is strain JG 31(T) ( = DSM 22788(T) = CCOS 200(T)).
一株革兰氏阳性、不规则杆状、非运动性、黄色素产生的细菌，菌株 JG31（T），是在鉴定耐铬土壤细菌的过程中分离得到的。对分离出的细菌进行 16S rRNA 基因序列分析，表明其在 Leucobacter 属内的系统发育位置。与当时已鉴定的 11 个 Leucobacter 属的种的二元 16S rRNA 基因序列比对表明，与 Leucobacter alluvii（GenBank 登录号：AM072820；99.4%）、Leucobacter iarius（AM040493；98.2%）、Leucobacter aridicollis（AJ781047；97.8%）、Leucobacter komagatae（AB007419；97.4%）、Leucobacter chironomi（EU346911；97.1%）和 Leucobacter luti（AM072819；97.1%）的相似性超过 97%。相比之下，DNA-DNA 杂交实验表明，与最密切相关的 Leucobacter alluvii、Leucobacter aridicollis 和 Leucobacter iarius 型菌株的 DNA 样本的相似度低于 28%。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的蛋白质分析和使用限制性内切酶 PvuII 的自动 RiboPrinting 将菌株 JG31（T）与 Leucobacter 属的所有型菌株区分开来。新型分离株的优势脂肪酸为anteiso-C（15：0）、anteiso-C（17：0）和 iso-C（16：0），而醌系统由menaquinones MK-11、MK-10、MK-9 和 MK-8 组成。在 B 型交联肽聚糖中，细胞壁氨基酸为丙氨酸、甘氨酸、苏氨酸、谷氨酸和 2,4-二氨基丁酸。菌株 JG31（T）能够在含有高达 300 mM K2CrO4 的培养基中生长，并在铬酸盐胁迫下表现出细胞聚集。基于生化和基因组分析，新菌株被认为代表 Leucobacter 属的一个新种，因此提议将其命名为 Leucobacter chromiiresistens sp. nov.。模式菌株是菌株 JG31（T）（=DSM 22788（T）=CCOS 200（T））。
