Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA.
mBio. 2010 Oct 12;1(4):e00208-10. doi: 10.1128/mBio.00208-10.
Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.
蝙蝠是新兴人畜共患病毒的宿主，这些病毒会对人类和动物健康产生深远影响，包括狂犬病病毒、丝状病毒、副黏病毒和严重急性呼吸综合征冠状病毒（SARS-CoV）。在一个专注于病原体发现的项目中，我们调查了在人类与蝙蝠接触可能促进病毒更有效种间传播的情况下，从尼日利亚洞穴中采集的蝙蝠的胃肠道组织。冠状病毒共识 PCR 和无偏高通量焦磷酸测序显示，在 Commerson's leaf-nosed bat（Hipposideros commersoni）中存在与 SARS-CoV 相关的冠状病毒序列。额外的基因组测序表明，与包括 SARS-CoV 在内的亚组 2b CoVs 不同，这种病毒是独特的，它在 M 和 N 基因之间包含三个重叠的开放阅读框，以及两个保守的茎环 II 基序。与这些特征相结合的系统发育分析表明，该病毒代表 2 型 CoV 中的一个新亚组。
