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精油对从犬类皮炎中分离出的[具体菌株,原文未完整给出]和[具体菌株,原文未完整给出]菌株的抗菌活性。

Antimicrobial Activity of Essential Oils against and Strains Isolated from Canine Dermatitis.

作者信息

Ebani Valentina Virginia, Bertelloni Fabrizio, Najar Basma, Nardoni Simona, Pistelli Luisa, Mancianti Francesca

机构信息

Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.

Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute", University of Pisa, via del Borghetto 80, 56124 Pisa, Italy.

出版信息

Microorganisms. 2020 Feb 13;8(2):252. doi: 10.3390/microorganisms8020252.

Abstract

spp. bacteria are the most frequently involved agents in canine cutaneous infections. Treatment of these infections is based on antibiotic therapy, that often is not effective because of the antibiotic-resistance of the bacterial strains. Cutaneous staphylococcal infections are often complicated by yeasts, that may be resistant to the conventional antifungal drugs. The present investigation was aimed to evaluate the in vitro antimicrobial activity of some essential oils (EOs) in view of a potential cutaneous application. In detail, EOs obtained from lemon verbena ( L'Hèr. Britton), cinnamon ( J. Presl), myrrh ( (Nees) Engl. var. ), lemongrass ( (DC.) Stapf), litsea ( (Lour.) Pers.), lemon balm ( L.), oregano ( L.), savory ( L.), and thyme ( L.) were assayed against spp. and strains previously isolated from dogs with dermatitis. All EOs were tested by agar disk diffusion and minimum inhibitory concentration methods to verify the antistaphylococcal activity, and by a microdilution method to evaluate the activity against . , and showed the best antibacterial activity against all the selected strains, with MICs ranging from 0.29 to 0.58 mg/mL, from 0.58 to 1.16 mg/mL, and from 0.56 to 1.12 mg/mL, respectively, whereas (1.03 mg/mL) and (1.8 mg/mL) were the most active against . After a proper in vivo evaluation, , , and EOs could be a promising treatment to combat canine cutaneous mixed infections.

摘要

葡萄球菌属细菌是犬类皮肤感染中最常涉及的病原体。这些感染的治疗基于抗生素疗法,但由于细菌菌株的抗生素耐药性,这种疗法往往无效。皮肤葡萄球菌感染常伴有酵母菌,这些酵母菌可能对传统抗真菌药物耐药。本研究旨在评估某些精油(EOs)在潜在皮肤应用方面的体外抗菌活性。具体而言,对从柠檬马鞭草(L'Hèr. Britton)、肉桂(J. Presl)、没药((Nees) Engl. var.)、柠檬草((DC.) Stapf)、山鸡椒((Lour.) Pers.)、柠檬香脂(L.)、牛至(L.)、夏香薄荷(L.)和百里香(L.)中提取的精油针对先前从患有皮炎的犬只分离出的葡萄球菌属和其他菌株进行了检测。所有精油均通过琼脂扩散法和最低抑菌浓度法检测以验证抗葡萄球菌活性,并通过微量稀释法评估其对其他菌株的活性。柠檬草、肉桂和百里香对所有选定菌株显示出最佳抗菌活性,其最低抑菌浓度分别为0.29至0.58毫克/毫升、0.58至1.16毫克/毫升和0.56至1.12毫克/毫升,而牛至(1.03毫克/毫升)和柠檬香脂(1.8毫克/毫升)对其他菌株活性最高。经过适当的体内评估后,柠檬草、肉桂和百里香精油可能是对抗犬类皮肤混合感染的一种有前景的治疗方法。

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