Li H, Xie S
Appl Opt. 1996 Apr 1;35(10):1793-5. doi: 10.1364/AO.35.001793.
A simple method based on total internal reflection is presented for measuring the refractive index of biological tissue. Anarrow laser beam and a semicylindrical lens in contact with a tissue specimen are used in the experimental apparatus. The dependence of the internal reflectivity on the angle of incidence is measured to determine the critical angle and therefore the refractive index of tissue. The experimental results for several tissue samples show that the method is reliable and useful for studying tissue optics. The principal advantages of the method are its elimination of scattering effects, suitability to a small in vitro sample, and excellent accuracy.
提出了一种基于全内反射的简单方法来测量生物组织的折射率。实验装置中使用了一束窄激光束和一个与组织样本接触的半圆柱形透镜。测量内反射率对入射角的依赖性，以确定临界角，从而确定组织的折射率。几个组织样本的实验结果表明，该方法对于研究组织光学是可靠且有用的。该方法的主要优点是消除了散射效应、适用于小的体外样本以及具有出色的准确性。
