Laboratoire d'Analyses, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne 5000 Monastir, Tunisia.
Microb Pathog. 2011 Jan;50(1):1-5. doi: 10.1016/j.micpath.2010.11.004. Epub 2010 Nov 18.
To analyze the degree of biofilm formation on three ica-positives Staphylococcus epidermidis as a function of biocides, the medium was supplemented with increasing concentrations of isopropanol, ethanol, and methanol at 0, 1, 4, 6, 8, 10, 12 and 14% (v/v), hydrogen peroxide (0, 0.125, 0.25, 0.5, 1, 2, 3, 4 and 5% v/v) and benzalkonium chloride (0, 0.125, 0.25, 0.5, 1, 2, 3, 4 and 6 μg ml⁻¹). In biocide-free biofilms, the results showed that two strains (S. epidermidis CIP106510 and E24) were strongly biofilm positive displaying a high oxidative activity (1.254 and 0.855, respectively) in comparison with the non-adherent one (S22). In addition biofilm formation was induced with 1% alcohol (isopropanol and ethanol) supplementation. The three studied strains cultured in TSB supplemented with 2% methanol displayed a strong oxidative activity (P=0.008). Moreover wells with 0.125% hydrogen peroxide enhanced increasing oxidative activity of S. epidermidis CIP106510 and S22. A significant induction of biofilm was noted after treatment with 1 μg ml⁻¹ of benzalkonium chloride. This study suggests that some biocides currently used in hospitals are ineffective against nosocomial pathogens growing in biofilms when used at weak concentration and fail to control this reservoir for hospital-acquired infection.
为了分析三种 ica 阳性表皮葡萄球菌生物膜形成的程度作为生物杀灭剂的功能，培养基中分别补充了不同浓度的异丙醇、乙醇和甲醇（0、1、4、6、8、10、12 和 14%（v/v））、过氧化氢（0、0.125、0.25、0.5、1、2、3、4 和 5%（v/v））和苯扎氯铵（0、0.125、0.25、0.5、1、2、3、4 和 6 μg/ml）。在无生物杀灭剂的生物膜中，结果表明两种菌株（表皮葡萄球菌 CIP106510 和 E24）的生物膜呈阳性，其氧化活性较高（分别为 1.254 和 0.855），与非黏附菌株（S22）相比。此外，补充 1%的酒精（异丙醇和乙醇）可以诱导生物膜形成。在补充有 2%甲醇的 TSB 中培养的三种研究菌株显示出很强的氧化活性（P=0.008）。此外，用 0.125%的过氧化氢处理可以增强表皮葡萄球菌 CIP106510 和 S22 的氧化活性。用 1μg/ml 的苯扎氯铵处理后，生物膜的诱导明显增强。本研究表明，目前在医院中使用的一些生物杀灭剂在弱浓度下对生物膜中生长的医院病原体无效，无法控制这种医院获得性感染的储库。
