University of Gothenburg, Department of Cell and Molecular Biology, Lundberg Laboratory, Medicinaregatan 9C, Göteborg, Sweden.
Curr Opin Biotechnol. 2011 Feb;22(1):94-102. doi: 10.1016/j.copbio.2010.10.013. Epub 2010 Nov 20.
Growth rate is an important variable and parameter in biology with a central role in evolutionary, functional genomics, and systems biology studies. In this review the pros and cons of the different technologies presently available for high-throughput measurements of growth rate are discussed. Growth rate can be measured in liquid microcultivation of individual strains, in competition between strains, as growing colonies on agar, as division of individual cells, and estimated from molecular reporters. Irrespective of methodology, statistical issues such as spatial biases and batch effects are crucial to investigate and correct for to ensure low false discovery rates. The rather low correlations between studies indicate that cross-laboratory comparison and standardization are pressing issue to assure high-quality and comparable growth-rate data.
增长率是生物学中的一个重要变量和参数，在进化、功能基因组学和系统生物学研究中起着核心作用。在这篇综述中，讨论了目前可用于高通量测量增长率的不同技术的优缺点。可以在液体微培养单个菌株、菌株竞争、琼脂上生长菌落、个体细胞分裂以及分子报告中测量增长率。无论采用何种方法，空间偏差和批次效应等统计问题对于进行调查和纠正以确保低错误发现率至关重要。研究之间相关性较低表明，跨实验室比较和标准化是确保高质量和可比增长率数据的紧迫问题。
