Facinelli B, Biavasco F, Varaldo P E
Institute of Microbiology, University of Ancona Medical School, Italy.
Eur J Epidemiol. 1990 Mar;6(1):80-3. doi: 10.1007/BF00155555.
DNA fingerprints of nine group C isolates of Neisseria meningitidis were obtained by digestion with three restriction endonucleases, BamHI, EcoRI, and HindIII. Identical restriction profiles were displayed by five strains isolated from two patients and three contacts during a localized outbreak of meningococcal disease in the area served by our laboratory. A slight difference, appreciable only in the fingerprints obtained with EcoRI, was observed between these isolates and a sixth one isolated from a clinical case occurring in the same area one year later. In contrast, three additional strains, recently isolated from patients in separate areas in Italy, gave restriction profiles differing strikingly from one another and from those produced by the strains isolated in our area.
通过用三种限制性内切酶BamHI、EcoRI和HindIII进行消化，获得了9株C群脑膜炎奈瑟菌分离株的DNA指纹图谱。在我们实验室服务区域内的一次局部脑膜炎球菌病暴发期间，从两名患者和三名接触者中分离出的5株菌株显示出相同的限制性图谱。在这些分离株与一年后从同一地区发生的临床病例中分离出的第六株菌株之间，观察到了细微差异，这种差异仅在用EcoRI获得的指纹图谱中明显。相比之下，最近从意大利不同地区的患者中分离出的另外3株菌株，其限制性图谱彼此之间以及与我们地区分离出的菌株所产生的图谱有显著差异。
