Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany.
Proteomics Clin Appl. 2010 Apr;4(4):464-78. doi: 10.1002/prca.200900189. Epub 2010 Feb 3.
Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well-characterized "real life" sample that can be used as reference standard in urine clinical proteomics studies.
We report on the generation of male and female urine samples that are extensively characterized by different platforms and methods (CE-MS, LC-MS, LC-MS/MS, 1-D gel analysis in combination with nano-LC MS/MS (using LTQ-FT ultra), and 2-DE-MS) for their proteome and peptidome. In several cases analysis involved a definition of the actual biochemical entities, i.e. proteins/peptides associated with molecular mass and detected PTMs and the relative abundance of these compounds.
The combination of different technologies allowed coverage of a wide mass range revealing the advantages and complementarities of the different technologies. Application of these samples in "inter-laboratory" and "inter-platform" data comparison is also demonstrated.
These well-characterized urine samples are freely available upon request to enable data comparison especially in the context of biomarker discovery and validation studies. It is also expected that they will provide the basis for the comprehensive characterization of the urinary proteome.
尿液蛋白质组学正成为一种强大的生物标志物发现工具。本研究的目的是开发一种经过充分特征描述的“真实生活”样本，可作为尿液临床蛋白质组学研究的参考标准。
我们报告了男性和女性尿液样本的生成，这些样本通过不同的平台和方法（CE-MS、LC-MS、LC-MS/MS、与纳升 LC-MS/MS（使用 LTQ-FT ultra）结合的 1-D 凝胶分析，以及 2-DE-MS）进行了广泛的蛋白质组学和肽组学特征描述。在某些情况下，分析涉及到实际生化实体的定义，即与分子量相关的蛋白质/肽以及检测到的 PTM 及其相对丰度。
不同技术的组合允许覆盖广泛的质量范围，揭示了不同技术的优势和互补性。还展示了这些样本在“实验室间”和“平台间”数据比较中的应用。
这些经过充分特征描述的尿液样本可根据要求免费提供，以促进数据比较，特别是在生物标志物发现和验证研究方面。预计它们还将为全面描述尿液蛋白质组学提供基础。
