大孔吸附树脂富集纯化荆芥总黄酮的研究
[Study on the enrichment and purification of total flavonoids in Schizonepeta tenuifolia by macroporous adsorption resin].
作者信息
Huang Zhi-Hong, Jiang Dong-Xu, Lai Xiao-Ping
机构信息
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
出版信息
Zhong Yao Cai. 2010 Sep;33(9):1476-80.
OBJECTIVE
To establish an optimum enrichment and purification process of total flavonoids in Schizonepeta tenuifolia by macroporous resins.
METHODS
9 kinds of resins were compared and the best one was chosen. Then the technique condition for separating and purifying total flavonoids from Schizonepeta tenuifolia was studied.
RESULTS
HP-20 was selected for its excellent adsorption and desorption properties, 80% ethanol was found to be the best elution solution. The best result was based on the follows: pH value-6.0, feeding rate-2 BV/h,sample concentration-0.4 g/mL, the ratio of total flavonoids to HP-20 macroporous resin-133. 76 mg/g, elution flowing rate-4 BV/h, eluting agent amount-3 BV. Then the purity reached 74. 31% and the yield was 97.13%.
CONCLUSION
The total flavonoids of Schizonepeta tenuifolia can be effectively purificated and separated by HP-20 macroporous resin.
目的
建立大孔树脂富集纯化荆芥总黄酮的最佳工艺。
方法
比较9种树脂，筛选出最佳树脂，研究其分离纯化荆芥总黄酮的工艺条件。
结果
HP-20树脂吸附和解吸性能优良，最佳洗脱剂为80%乙醇。最佳工艺条件为：pH值6.0，上样速率2 BV/h，样品浓度0.4 g/mL，总黄酮与HP-20大孔树脂质量比133.76 mg/g，洗脱流速4 BV/h，洗脱剂用量3 BV。纯化后总黄酮纯度达74.31%，得率为97.13%。
结论
HP-20大孔树脂可有效分离纯化荆芥总黄酮。
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