Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002 South Africa.
Mycologia. 2004 Jul-Aug;96(4):866-78. doi: 10.1080/15572536.2005.11832933.
The genus Ophiostoma includes numerous species of primarily insect-vectored, wood-staining fungi. Several anamorph genera that differ in their micronematous or macronematous conidiogenous cells have been associated with Ophiostoma species. Among the former group, Sporothrix is associated with many species and is characterized by conidiogenous cells that arise laterally or terminally from any place on the hyphae and produce nonseptate conidia on sympodially developing denticles. The purpose of this study was to characterize ophiostomatoid isolates with Sporothrix anamorphs recently collected in Austria and Azerbaijan. The isolates were characterized based on comparisons of rDNA and β-tubulin sequence data. Morphology, growth in culture, and sexual reproductive mode were also considered. Phylogenetic analyses of the combined sequence data showed that the isolates formed two distinct groups, one including isolates from Austria and the other isolates from Austria and Azerbaijan. Growth at 25 C and morphology revealed some differences between the two groups, and supported the view that they represent two new species, which we describe here as Ophiostoma fusiforme sp. nov. and Ophiostoma lunatum sp. nov. Both these groups phylogenetically were related to, but distinct from, Ophiostoma stenoceras.
束梗孢属包含许多主要通过昆虫传播、木质变色的真菌种。与 Ophiostoma 种相关的一些无性型属在其微菌核或大菌核的产孢细胞上存在差异。在前一组中，Sporothrix 与许多种相关，其特征是产孢细胞从菌丝的任何部位侧向或末端生出，在共生发育的齿突上产生无隔膜的分生孢子。本研究的目的是描述最近在奥地利和阿塞拜疆收集的具有 Sporothrix 无性型的束梗孢属分离物。这些分离物是基于 rDNA 和 β-微管蛋白序列数据的比较来进行特征描述的。形态学、培养中的生长和有性生殖方式也被考虑在内。联合序列数据分析显示，这些分离物形成了两个不同的组，一组包括来自奥地利的分离物，另一组包括来自奥地利和阿塞拜疆的分离物。在 25°C 下的生长和形态学揭示了这两个组之间的一些差异，并支持它们代表两个新种的观点，我们在这里将它们描述为 Ophiostoma fusiforme sp. nov. 和 Ophiostoma lunatum sp. nov. 这两个组在系统发育上与 Ophiostoma stenoceras 相关，但与 Ophiostoma stenoceras 不同。
