Buchbauer G, Jirovetz L, Jäger W, Plank C, Dietrich H
Institute of Pharmaceutical Chemistry, University of Vienna, Austria.
J Pharm Sci. 1993 Jun;82(6):660-4. doi: 10.1002/jps.2600820623.
Fragrance compounds and essential oils with sedative effects influence the motility of mice in inhalation studies under standardized conditions. A significant drop in the motility of mice was registered following exposure to these fragrances. The same results were achieved when the mice were artificially induced into overagitation by intraperitoneal application of caffeine and subsequently subjected to inhalation of fragrance compounds and essential oils. These results proved the sedative effects of these fragrants via inhalative exposure in low concentrations. Blood samples were taken from the mice after a 1-h inhalation period. Chromatographic and spectroscopic methods were used to detect and characterize the actual effective compounds after solid-phase extraction. Serum concentrations of 42 different substances, including fragrance compounds, were found in low ranges (ng/mL serum). The results contribute to the correct interpretation of the term aromatherapy (i.e., a stimulating or sedative effect on the behaviour of individuals only upon inhalation of fragrance compounds).
在标准化条件下的吸入研究中，具有镇静作用的香料化合物和精油会影响小鼠的活动能力。接触这些香料后，小鼠的活动能力显著下降。当通过腹腔注射咖啡因人为诱导小鼠过度兴奋，随后使其吸入香料化合物和精油时，也得到了相同的结果。这些结果证明了低浓度吸入这些香料具有镇静作用。在吸入1小时后从小鼠身上采集血样。采用色谱和光谱方法在固相萃取后检测并鉴定实际有效的化合物。发现包括香料化合物在内的42种不同物质的血清浓度处于低水平（纳克/毫升血清）。这些结果有助于正确解释芳香疗法这一术语（即仅在吸入香料化合物时对个体行为产生刺激或镇静作用）。
